促性腺激素释放激素：对体外灌注兔卵巢的影响

Gonadotropin-releasing hormone: effects on the in vitro perfused rabbit ovary.

作者信息

Eisenberg E, Kitai H, Kobayashi Y, Santulli R, Wallach E E

出版信息

Biol Reprod. 1984 Jun;30(5):1216-21. doi: 10.1095/biolreprod30.5.1216.

DOI:10.1095/biolreprod30.5.1216

Abstract

Gonadotropin-releasing hormone (GnRH) has been shown to inhibit ovulation in gonadotropin-primed hypophysectomized rats and steroid production in cultured rat granulosa cells. To determine if similar effects of GnRH can be observed in another species, the extracorporeal perfused rabbit ovary was utilized. Two groups of rabbit ovaries were exposed to GnRH in a pulsatile fashion at two dose levels (Group I, 2.56 X 10(-8) M; Group II, 2.56 X 10(-7) M). Contralateral ovaries were not perfused with GnRH. Human chorionic gonadotropin (hCG) was added to the perfusate of all ovaries 30 min after the onset of perfusion. Ovulation occurred in all ovaries exposed to hCG in the presence or absence of GnRH. Ovulatory efficiency was similar in both the experimental and control groups. No statistical difference could be determined in the time of ovulation, stage of maturity of oocytes, or percent of degeneration of ovulated or follicular oocytes. Progesterone production was not inhibited in the GnRH-treated ovaries. In contrast to observations in the rat, GnRH does not exhibit a direct inhibitory effect on ovulation or steroid production in the rabbit.

摘要

促性腺激素释放激素（GnRH）已被证明可抑制促性腺激素预处理的垂体切除大鼠的排卵，并抑制培养的大鼠颗粒细胞中的类固醇生成。为了确定在另一种物种中是否能观察到GnRH的类似作用，使用了体外灌注的兔卵巢。两组兔卵巢以两种剂量水平接受脉冲式GnRH处理（第一组，2.56×10⁻⁸ M；第二组，2.56×10⁻⁷ M）。对侧卵巢未用GnRH灌注。灌注开始30分钟后，向所有卵巢的灌注液中加入人绒毛膜促性腺激素（hCG）。无论有无GnRH，所有暴露于hCG的卵巢均发生排卵。实验组和对照组的排卵效率相似。在排卵时间、卵母细胞成熟阶段或排卵或卵泡卵母细胞的退化百分比方面，未发现统计学差异。GnRH处理的卵巢中孕酮生成未受抑制。与在大鼠中的观察结果相反，GnRH对兔的排卵或类固醇生成没有直接抑制作用。