Cleary M L, Wood G S, Warnke R, Chao J, Sklar J
Blood. 1984 Jul;64(1):99-104.
Studies of hairy cell leukemia have yielded conflicting data about the cell of origin in this disease. To investigate this issue, we have examined the state of immunoglobulin genes in the cells of 11 randomly selected spleens showing histologic involvement with hairy cell leukemia. DNA was extracted from splenic tissue samples and digested with restriction endonucleases. Following agarose gel electrophoresis and transfer to nitrocellulose filters or activated nylon membranes, splenic DNA was hybridized with radiolabeled DNA fragment probes specific for the constant regions of the immunoglobulin heavy chain and kappa and lambda light chain genes. Autoradiograms of the hybridized DNA in each case revealed rearrangements of a heavy chain gene and at least one light chain gene. In addition, immunophenotyping of cellular immunoglobulin polypeptides was carried out on frozen tissue sections from all but one case. In each case in which an immunoglobulin polypeptide could be detected, a rearrangement was present in the DNA of the corresponding immunoglobulin gene. These studies offer strong evidence for endogenous immunoglobulin synthesis in hairy cells and for the B lymphocytic character of this leukemia.
毛细胞白血病的研究就该疾病的细胞起源得出了相互矛盾的数据。为了研究这个问题，我们检查了11个随机选取的、显示有毛细胞白血病组织学累及的脾脏细胞中免疫球蛋白基因的状态。从脾脏组织样本中提取DNA，并用限制性内切酶进行消化。在琼脂糖凝胶电泳并转移至硝酸纤维素滤膜或活化尼龙膜后，脾脏DNA与针对免疫球蛋白重链恒定区以及κ和λ轻链基因的放射性标记DNA片段探针进行杂交。每种情况下杂交DNA的放射自显影片均显示重链基因和至少一个轻链基因发生了重排。此外，除了1例之外，对所有病例的冷冻组织切片进行了细胞免疫球蛋白多肽的免疫表型分析。在每例能够检测到免疫球蛋白多肽的病例中，相应免疫球蛋白基因的DNA中均存在重排。这些研究为毛细胞内源性免疫球蛋白合成以及该白血病的B淋巴细胞特征提供了有力证据。
