Milner J D, Wurtman R J
Brain Res. 1984 May 28;301(1):139-42. doi: 10.1016/0006-8993(84)90410-4.
An experimental system is described for measuring the release of endogenous dopamine from electrically stimulated slices of rat striatum. Striatal slices were field-stimulated by two high frequency trains (S1 and S2) applied 10, 30 or 60 min apart. The quantities of dopamine released by the two stimuli were compared from slices incubated with and without dopamine's precursor, L-tyrosine. Sustained release of dopamine evoked by the two stimuli was shown to require the inclusion of tyrosine (50 microM) in the superfusate.
描述了一种用于测量大鼠纹状体电刺激切片中内源性多巴胺释放的实验系统。纹状体切片通过间隔10、30或60分钟施加的两列高频刺激（S1和S2）进行场刺激。比较了在有和没有多巴胺前体L-酪氨酸孵育的切片中两种刺激释放的多巴胺量。结果表明，两种刺激诱发的多巴胺持续释放需要在灌流液中加入酪氨酸（50微摩尔）。
