Nishida T, Sakamoto S, Yamamoto T, Hayman M, Kawai S, Toyoshima K
Gan. 1984 Apr;75(4):325-33.
The genomic DNAs of two strains of avian erythroblastosis virus (AEV), AEV-R and AEV-H, were molecularly cloned in Escherichia coli using lambda Charon 16A as a vector. Comparison of the restriction maps of the cloned DNAs with that of AEV-ES4 DNA revealed that R strain is identical with ES4 strain, but totally differs from H strain. The erbB gene product of AEV-R and that of AEV-H were shown to be glycoproteins with molecular weights of 68,000 and 72,000, respectively. Nucleotide sequence analysis of the 3' part of the erbB gene showed that the erbB gene in AEV-R is 126 base pairs (bp) shorter than that in AEV-H, which might result in some difference in tumorigenicity between these viruses.
利用λ噬菌体Charon 16A作为载体，将两株禽成红细胞增多症病毒（AEV），即AEV-R和AEV-H的基因组DNA在大肠杆菌中进行了分子克隆。将克隆DNA的限制性图谱与AEV-ES4 DNA的图谱进行比较，结果显示R株与ES4株相同，但与H株完全不同。AEV-R和AEV-H的erbB基因产物被证明是糖蛋白，分子量分别为68,000和72,000。erbB基因3'部分的核苷酸序列分析表明，AEV-R中的erbB基因比AEV-H中的短126个碱基对（bp），这可能导致这些病毒在致瘤性上存在一些差异。
