Miyajima N, Kadowaki Y, Fukushige S, Shimizu S, Semba K, Yamanashi Y, Matsubara K, Toyoshima K, Yamamoto T
Institute of Medical Science, University of Tokyo, Japan.
Nucleic Acids Res. 1988 Dec 9;16(23):11057-74. doi: 10.1093/nar/16.23.11057.
Two v-erbA-related genes, named ear-2 and ear-3, have been identified in the human genome and characterized by cDNA cloning. These genes are predicted to encode proteins that are very similar in primary structure to receptors for steroid hormones or thyroid hormone (T3). In addition, amino acid sequences of the ear-2 and ear-3 gene products are very similar each other especially at the DNA binding domain (86% homology) and at the putative ligand binding domain (76% homology). Northern hybridization with ear DNA probes of RNAs from various tissues of a human fetus reveals that the expression of ear-2 is high in the liver whereas the expression of ear-3 is relatively ubiquitous. Hybridization analysis of DNAs from sorted chromosomes shows that the ear-2 gene is located on chromosome 19 and ear-3 on chromosome 5, indicating that the two genes are clearly different from each other.
在人类基因组中已鉴定出两个与v-erbA相关的基因，分别命名为ear-2和ear-3，并通过cDNA克隆对其进行了表征。预计这些基因编码的蛋白质在一级结构上与类固醇激素或甲状腺激素（T3）的受体非常相似。此外，ear-2和ear-3基因产物的氨基酸序列彼此非常相似，特别是在DNA结合结构域（86%同源性）和假定的配体结合结构域（76%同源性）。用人胎儿各种组织的RNA与ear DNA探针进行Northern杂交分析表明，ear-2在肝脏中的表达较高，而ear-3的表达相对普遍。对分选染色体的DNA进行杂交分析表明，ear-2基因位于19号染色体上，ear-3基因位于5号染色体上，这表明这两个基因彼此明显不同。
