Nilsson K, Klareskog L, Ralph P, Sundström C, Zech L
Hematol Oncol. 1983 Oct-Dec;1(4):277-95. doi: 10.1002/hon.2900010402.
A new Epstein-Barr virus nuclear antigen (EBNA) negative cell line SKW 4 has been established in vitro from a patient with diffuse histiocytic lymphoma. The SKW 4 seems to be an authentic human tumour cell line as evidenced by its EBV negativity, monoclonality and aneuploidy tested during early in vitro passage. The cell line expresses surface mu and kappa-chains, HLA-DR antigen, C3 and Fc receptors and B-cell lineage antigens. The karyotypic analyses demonstrated many numerical and structural aberrations. No Burkitt lymphoma associated translocations (t8;14, t2;8, t;22) were detected, but most of the markers found are those commonly associated with various types of human cancer. The SKW 4 thus represents the most common type of 'histiocytic lymphoma', that with a B-lymphoid cell phenotype, but is unique among HL derived lymphoma lines in its strong expression of a Helix pomatia A agglutinin binding surface glycoprotein of an apparent molecular weight of 75 000 daltons.
已从一名弥漫性组织细胞淋巴瘤患者体内体外建立了一种新的爱泼斯坦 - 巴尔病毒核抗原（EBNA）阴性细胞系SKW 4。SKW 4似乎是一种真正的人类肿瘤细胞系，在早期体外传代过程中通过EBV阴性、单克隆性和非整倍体检测得到证实。该细胞系表达表面μ链和κ链、HLA - DR抗原、C3和Fc受体以及B细胞系抗原。核型分析显示出许多数量和结构异常。未检测到伯基特淋巴瘤相关的易位（t8;14、t2;8、t;22），但发现的大多数标记物是那些通常与各种类型人类癌症相关的标记物。因此，SKW 4代表了“组织细胞淋巴瘤”最常见的类型，即具有B淋巴细胞表型的类型，但在源自HL的淋巴瘤细胞系中，它独特之处在于强烈表达一种明显分子量为75000道尔顿的苹果螺凝集素结合表面糖蛋白。
