Kamesaki H, Miwa H, Ohno Y, Miyanishi S, Yamabe H, Doi S, Arita Y, Ohno H, Tatsumi E, Nishikori M
Department of Internal Medicine, Faculty of Medicine, Kyoto University.
Jpn J Cancer Res. 1988 Nov;79(11):1193-200. doi: 10.1111/j.1349-7006.1988.tb01544.x.
A novel cell line, designated KIS-1, was established from a patient with Ki-1-positive diffuse large cell lymphoma. Multiple phenotypic analysis of the KIS-1 cells was carried out with a total of 22 monoclonal antibodies defining hematopoietic cell subsets and lineages. The KIS-1 cells were positive for Ki-1, B4, HLA-DR, and 2D1 (common leucocyte) antigens, but were negative for the antigens reportedly specific for T cells, natural killer cells, granulocytes, monocytes, interdigitating reticulum cells and dendritic reticulum cells. The genomic analysis of the KIS-1 cells showed not only the rearrangement of JH and J kappa genes but also the probable rearrangement of C lambda genes. Moreover, the cells produced immunoglobulin lambda chains. Thus, KIS-1 was considered to be of B-cell lineage. The lymphoma-cell derivation of KIS-1 was based on the following facts. The cytochemical, immunologic, cytogenetic properties and the results of the molecular genomic analysis in the KIS-1 cells were essentially the same as those of the original tumor cells, and the KIS-1 cells were negative for Epstein-Barr virus-associated nuclear antigen. KIS-1 is the only known B-cell line derived from Ki-1-positive diffuse large cell lymphoma, and should be useful for defining the biological implications of Ki-1 antigen.
一种名为KIS-1的新型细胞系是从一名患有Ki-1阳性弥漫性大细胞淋巴瘤的患者身上建立的。使用总共22种定义造血细胞亚群和谱系的单克隆抗体对KIS-1细胞进行了多种表型分析。KIS-1细胞对Ki-1、B4、HLA-DR和2D1（普通白细胞）抗原呈阳性，但对据报道对T细胞、自然杀伤细胞、粒细胞、单核细胞、交错突网状细胞和树突状网状细胞特异的抗原呈阴性。KIS-1细胞的基因组分析不仅显示了JH和Jκ基因的重排，还显示了Cλ基因可能的重排。此外，这些细胞产生免疫球蛋白λ链。因此，KIS-1被认为是B细胞谱系。KIS-1的淋巴瘤细胞起源基于以下事实。KIS-1细胞的细胞化学、免疫学、细胞遗传学特性以及分子基因组分析结果与原始肿瘤细胞基本相同，并且KIS-1细胞的爱泼斯坦-巴尔病毒相关核抗原呈阴性。KIS-1是唯一已知的源自Ki-1阳性弥漫性大细胞淋巴瘤的B细胞系，应该有助于确定Ki-1抗原的生物学意义。
