Cam Y, Bellon G, Poulin G, Caron Y, Birembaut P
Invasion Metastasis. 1984;4(2):61-72.
The distribution of type IV collagen in benign and malignant epithelial proliferations of the breasts, the lungs, and the skin was studied by an indirect immunofluorescence technique using specific antiserum. In benign lesions of the breasts, the staining for type IV collagen was present in all vascular and glandular basement membranes. In basal cell carcinoma of the skin, the basement membrane labeling was also found to be continuous. In malignant lesions of the breasts, the lungs, and the skin, staining for type IV collagen was seen only around well-differentiated glandular structures and in close contact to basal epidermal cells. This staining appeared as an irregular network. Of particular interest was the localization of type IV collagen in non-infiltrating lesions of the breasts and the bronchi where discontinuity in the basement membrane staining was observed. In contrast, there were no disruptions of basement membrane labeling in skin senile keratosis and in Bowen's disease. We conclude that the loss of type IV collagen seen in malignant proliferations in our study is related to overt or potential tumor cell infiltration and aggressiveness.
利用特异性抗血清，通过间接免疫荧光技术研究了IV型胶原在乳腺、肺和皮肤的良性及恶性上皮增生中的分布情况。在乳腺良性病变中，IV型胶原染色存在于所有血管和腺泡基底膜中。在皮肤基底细胞癌中，也发现基底膜标记是连续的。在乳腺、肺和皮肤的恶性病变中，IV型胶原染色仅见于分化良好的腺泡结构周围以及与表皮基底细胞紧密接触处。这种染色表现为不规则网络状。特别令人感兴趣的是IV型胶原在乳腺和支气管非浸润性病变中的定位，在这些病变中观察到基底膜染色不连续。相比之下，皮肤老年角化病和鲍温病中基底膜标记没有中断。我们得出结论，在我们的研究中，恶性增殖中所见的IV型胶原缺失与明显的或潜在的肿瘤细胞浸润及侵袭性有关。
