Birembaut P, Caron Y, van Cauwenberge D, Foidart J M
Coll Relat Res. 1983;3(1):25-31.
Distribution of laminin, a basement membrane glycoprotein, was studied by an indirect immunofluorescence technique in benign, dysplastic and malignant lesions of breast, lungs and uterine cervix as compared with normal tissues. Laminin was present with a linear and continuous staining in epithelial and vascular basement membranes in normal tissues, benign and dysplastic lesions and in in situ carcinomas of the uterine cervix. By contrast, laminin was absent or very irregularly distributed around malignant infiltrating tumoral clusters. Of particular interest was the linear and disrupted labeling observed in intra-epithelial carcinomas of breast and bronchi. Loss of laminin in these conditions could be related to a potential tumoral invasion.
采用间接免疫荧光技术，研究了层粘连蛋白（一种基底膜糖蛋白）在乳腺、肺和子宫颈的良性、发育异常及恶性病变中的分布情况，并与正常组织进行了比较。在正常组织、良性和发育异常病变以及子宫颈原位癌的上皮和血管基底膜中，层粘连蛋白呈现线性且连续的染色。相比之下，在恶性浸润性肿瘤团块周围，层粘连蛋白缺失或分布极不规则。特别值得关注的是在乳腺和支气管上皮内癌中观察到的线性且中断的标记。在这些情况下，层粘连蛋白的缺失可能与潜在的肿瘤侵袭有关。
