枯草芽孢杆菌中芽孢形成基因spoIIC的克隆

Cloning of sporulation gene spoIIC in Bacillus subtilis.

作者信息

Anaguchi H, Fukui S, Shimotsu H, Kawamura F, Saito H, Kobayashi Y

出版信息

J Gen Microbiol. 1984 Apr;130(4):757-60. doi: 10.1099/00221287-130-4-757.

Abstract

Specialized transducing phages rho 11spoIIC and phi 105spoIIC, carrying the Bacillus subtilis sporulation gene spoIIC, were constructed by the prophage transformation method. An EcoRI fragment (2.4 MDal) carrying the spoIIC gene was isolated from the phi 105spoIIC genome and recloned into the EcoRI site of plasmid pUB110. The recombinant plasmids corrected the sporulation defect of a Spo- Rec- host, but slightly inhibited the sporulation of a Spo+ Rec- host.

摘要

通过原噬菌体转化法构建了携带枯草芽孢杆菌芽孢形成基因spoIIC的特异性转导噬菌体rho 11spoIIC和phi 105spoIIC。从phi 105spoIIC基因组中分离出携带spoIIC基因的EcoRI片段（2.4 MDal），并将其重新克隆到质粒pUB110的EcoRI位点。重组质粒纠正了Spo- Rec-宿主的芽孢形成缺陷，但略微抑制了Spo+ Rec-宿主的芽孢形成。