枯草芽孢杆菌spoIVCA突变的抑制突变分析：在缺乏位点特异性DNA重组酶SpoIVCA的情况下DNA重排的发生
Analysis of suppressor mutations of spoIVCA mutations: occurrence of DNA rearrangement in the absence of site-specific DNA recombinase SpoIVCA in Bacillus subtilis.
作者信息
Sato T, Harada K, Kobayashi Y
机构信息
Department of Applied Biological Science, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Japan.
出版信息
J Bacteriol. 1996 Jun;178(11):3380-3. doi: 10.1128/jb.178.11.3380-3383.1996.
Abstract
The spoIVCA gene of Bacillus subtilis encodes a site-specific recombinase, which excises a 48-kb skin element from the chromosomal DNA by DNA rearrangement and creates a new composite gene, sigK, on the chromosome. From spoIVCA mutants, we have isolated Spo+ revertants which have no skin element but have an intact sigK gene. This result suggests that the DNA rearrangement can occur in the absence of spoIVCA.
摘要
枯草芽孢杆菌的spoIVCA基因编码一种位点特异性重组酶，该酶通过DNA重排从染色体DNA中切除一个48kb的间隔元件，并在染色体上产生一个新的复合基因sigK。从spoIVCA突变体中，我们分离出了Spo+回复体，它们没有间隔元件，但有完整的sigK基因。这一结果表明，DNA重排在没有spoIVCA的情况下也能发生。
相似文献
1
Analysis of suppressor mutations of spoIVCA mutations: occurrence of DNA rearrangement in the absence of site-specific DNA recombinase SpoIVCA in Bacillus subtilis.枯草芽孢杆菌spoIVCA突变的抑制突变分析：在缺乏位点特异性DNA重组酶SpoIVCA的情况下DNA重排的发生
J Bacteriol. 1996 Jun;178(11):3380-3. doi: 10.1128/jb.178.11.3380-3383.1996.
2
Expression of the Bacillus subtilis spoIVCA gene, which encodes a site-specific recombinase, depends on the spoIIGB product.编码位点特异性重组酶的枯草芽孢杆菌spoIVCA基因的表达取决于spoIIGB产物。
J Bacteriol. 1994 Feb;176(3):935-7. doi: 10.1128/jb.176.3.935-937.1994.
3
Binding of the Bacillus subtilis spoIVCA product to the recombination sites of the element interrupting the sigma K-encoding gene.枯草芽孢杆菌spoIVCA产物与中断编码σK基因的元件的重组位点的结合。
Proc Natl Acad Sci U S A. 1992 Jul 1;89(13):5991-5. doi: 10.1073/pnas.89.13.5991.
4
The Bacillus subtilis gene for the development transcription factor sigma K is generated by excision of a dispensable DNA element containing a sporulation recombinase gene.枯草芽孢杆菌中负责发育转录因子σK的基因是通过切除一个含有芽孢形成重组酶基因的可去除DNA元件而产生的。
Genes Dev. 1990 Apr;4(4):525-35. doi: 10.1101/gad.4.4.525.
5
Anabaena xisF gene encodes a developmentally regulated site-specific recombinase.鱼腥藻xisF基因编码一种受发育调控的位点特异性重组酶。
Genes Dev. 1994 Jan;8(1):74-83. doi: 10.1101/gad.8.1.74.
6
The ripX locus of Bacillus subtilis encodes a site-specific recombinase involved in proper chromosome partitioning.枯草芽孢杆菌的ripX基因座编码一种参与正确染色体分配的位点特异性重组酶。
J Bacteriol. 1999 Oct;181(19):6053-62. doi: 10.1128/JB.181.19.6053-6062.1999.
7
The cisA cistron of Bacillus subtilis sporulation gene spoIVC encodes a protein homologous to a site-specific recombinase.枯草芽孢杆菌芽孢形成基因spoIVC的顺式A顺反子编码一种与位点特异性重组酶同源的蛋白质。
J Bacteriol. 1990 Feb;172(2):1092-8. doi: 10.1128/jb.172.2.1092-1098.1990.
8
Identification and characterization of int (integrase), xis (excisionase) and chromosomal attachment sites of the integrative and conjugative element ICEBs1 of Bacillus subtilis.枯草芽孢杆菌整合性接合元件ICEBs1的整合酶（int）、切除酶（xis）及染色体附着位点的鉴定与表征
Mol Microbiol. 2007 Dec;66(6):1356-69. doi: 10.1111/j.1365-2958.2007.06000.x. Epub 2007 Nov 13.
9
Effects of replication termination mutants on chromosome partitioning in Bacillus subtilis.复制终止突变体对枯草芽孢杆菌染色体分配的影响。
Proc Natl Acad Sci U S A. 2001 Jan 2;98(1):212-7. doi: 10.1073/pnas.98.1.212.
10
The Bacillus subtilis chromatin-associated protein Hbsu is involved in DNA repair and recombination.枯草芽孢杆菌染色质相关蛋白Hbsu参与DNA修复和重组。
Mol Microbiol. 1997 Mar;23(6):1169-79. doi: 10.1046/j.1365-2958.1997.3061670.x.
引用本文的文献
1
New candidates for regulated gene integrity revealed through precise mapping of integrative genetic elements.通过精确绘制整合遗传元件，揭示了新的受调控基因完整性候选物。
Nucleic Acids Res. 2020 May 7;48(8):4052-4065. doi: 10.1093/nar/gkaa156.
2
A new perspective on lysogeny: prophages as active regulatory switches of bacteria.一种关于溶原性的新视角：噬菌体作为细菌的活跃调控开关。
Nat Rev Microbiol. 2015 Oct;13(10):641-50. doi: 10.1038/nrmicro3527.
3
Repression of sigK intervening (skin) element gene expression by the CI-like protein SknR and effect of SknR depletion on growth of Bacillus subtilis cells.CI 样蛋白 SknR 对 sigK 插入（皮肤）元件基因表达的抑制作用及 SknR 缺失对枯草芽孢杆菌细胞生长的影响。
J Bacteriol. 2010 Dec;192(23):6209-16. doi: 10.1128/JB.00625-10. Epub 2010 Oct 1.
4
spoIVH (ykvV), a requisite cortex formation gene, is expressed in both sporulating compartments of Bacillus subtilis.spoIVH（ykvV）是一种必需的皮层形成基因，在枯草芽孢杆菌的两个芽孢形成区室中均有表达。
J Bacteriol. 2004 Aug;186(16):5450-9. doi: 10.1128/JB.186.16.5450-5459.2004.
5
Compartmentalization of gene expression during Bacillus subtilis spore formation.枯草芽孢杆菌孢子形成过程中基因表达的区室化
Microbiol Mol Biol Rev. 2004 Jun;68(2):234-62. doi: 10.1128/MMBR.68.2.234-262.2004.
6
Analysis of the Bacillus subtilis spoIIIJ gene and its Paralogue gene, yqjG.枯草芽孢杆菌spoIIIJ基因及其旁系同源基因yqjG的分析
J Bacteriol. 2002 Apr;184(7):1998-2004. doi: 10.1128/JB.184.7.1998-2004.2002.
7
The ars operon in the skin element of Bacillus subtilis confers resistance to arsenate and arsenite.枯草芽孢杆菌皮肤元件中的ars操纵子赋予对砷酸盐和亚砷酸盐的抗性。
J Bacteriol. 1998 Apr;180(7):1655-61. doi: 10.1128/JB.180.7.1655-1661.1998.
本文引用的文献
1
Two tRNA gene clusters associated with rRNA operons rrnD and rrnE in Bacillus subtilis.枯草芽孢杆菌中与rRNA操纵子rrnD和rrnE相关的两个tRNA基因簇。
J Bacteriol. 1993 Jan;175(2):503-9. doi: 10.1128/jb.175.2.503-509.1993.
2
Expression of the Bacillus subtilis spoIVCA gene, which encodes a site-specific recombinase, depends on the spoIIGB product.编码位点特异性重组酶的枯草芽孢杆菌spoIVCA基因的表达取决于spoIIGB产物。
J Bacteriol. 1994 Feb;176(3):935-7. doi: 10.1128/jb.176.3.935-937.1994.
3
Anabaena xisF gene encodes a developmentally regulated site-specific recombinase.鱼腥藻xisF基因编码一种受发育调控的位点特异性重组酶。
Genes Dev. 1994 Jan;8(1):74-83. doi: 10.1101/gad.8.1.74.
4
Complete nucleotide sequence of a skin element excised by DNA rearrangement during sporulation in Bacillus subtilis.
Microbiology (Reading). 1995 Feb;141 ( Pt 2):323-7. doi: 10.1099/13500872-141-2-323.
5
The Bacillus subtilis ochre suppressor sup-3 is located in an operon of seven tRNA genes.枯草芽孢杆菌赭石色抑制基因sup-3位于一个由七个tRNA基因组成的操纵子中。
J Bacteriol. 1993 Oct;175(20):6512-7. doi: 10.1128/jb.175.20.6512-6517.1993.
6
Cloning of sporulation gene spoIIC in Bacillus subtilis.枯草芽孢杆菌中芽孢形成基因spoIIC的克隆
J Gen Microbiol. 1984 Apr;130(4):757-60. doi: 10.1099/00221287-130-4-757.
7
Catabolic repression of bacterial sporulation.细菌芽孢形成的分解代谢阻遏
Proc Natl Acad Sci U S A. 1965 Sep;54(3):704-11. doi: 10.1073/pnas.54.3.704.
8
Phenotypic and genetic characterization of mutations in the spoIVC locus of Bacillus subtilis.枯草芽孢杆菌spoIVC基因座突变的表型和遗传特征分析
J Gen Microbiol. 1988 Jan;134(1):9-17. doi: 10.1099/00221287-134-1-9.
9
Transcriptional regulation and structure of the Bacillus subtilis sporulation locus spoIIIC.枯草芽孢杆菌芽孢形成基因座spoIIIC的转录调控与结构
J Bacteriol. 1988 Mar;170(3):1162-7. doi: 10.1128/jb.170.3.1162-1167.1988.
10
The promoter for a sporulation gene in the spoIVC locus of Bacillus subtilis and its use in studies of temporal and spatial control of gene expression.枯草芽孢杆菌spoIVC基因座中一个芽孢形成基因的启动子及其在基因表达时空控制研究中的应用。
J Bacteriol. 1988 Aug;170(8):3513-22. doi: 10.1128/jb.170.8.3513-3522.1988.
Zotero 插件