血液中β-内啡肽样免疫反应性的两个池：血浆和红细胞。

Two pools of beta-endorphin-like immunoreactivity in blood: plasma and erythrocytes.

作者信息

Fisher A, Comly M, Do R, Tamarkin L, Ghazanfari A F, Mukherjee A B

出版信息

Life Sci. 1984 May 7;34(19):1839-46. doi: 10.1016/0024-3205(84)90677-5.

Abstract

While attempting to delineate the reason for the reported extreme variability of beta-endorphin-like immunoreactivity (beta-ir) in human plasma (eg., nondetectable to 1 ng/ml) by standard radioimmunoassay, we noted that a substantial portion of circulating beta-ir was associated with erythrocytes. That erythrocyte associated beta-ir is authentic beta-endorphin (beta-EP) was confirmed by high performance liquid chromatography (HPLC). Analysis of blood samples from rabbits, rats and mice revealed the presence of beta-ir in erythrocytes from these species as well. These results suggest that there are two pools of beta-endorphin-like immunoreactivity in blood: plasma and erythrocytes.

摘要

在试图通过标准放射免疫测定法来阐明所报道的人血浆中β-内啡肽样免疫反应性（β-ir）极端变异性（例如，从检测不到到1 ng/ml）的原因时，我们注意到循环中的β-ir有很大一部分与红细胞相关。高效液相色谱法（HPLC）证实与红细胞相关的β-ir是真正的β-内啡肽（β-EP）。对来自兔子、大鼠和小鼠的血样分析表明，这些物种的红细胞中也存在β-ir。这些结果表明，血液中存在两种β-内啡肽样免疫反应性池：血浆和红细胞。