Yamamoto H
Microbiol Immunol. 1984;28(3):339-48. doi: 10.1111/j.1348-0421.1984.tb00685.x.
Two types of glycoproteins were isolated from the membrane fraction of adenovirus type 12 (Ad12) hamster tumor cells by recovering detergent-solubilized glycoproteins using concanavalin A-affinity chromatography and gel filtration. One of the glycoproteins consisted of a polypeptide of 130,000 daltons (130K) with a pI value of 4.7-5.1, and the other consisted of a polypeptide of 18,500 daltons (18.5K) with a pI value of 6.3-6.6. The glycoproteins were immunologically different. The 18.5K glycoprotein induced in vivo resistance to tumor growth and anti-tumor cytotoxic T cells, while the 130K glycoprotein induced in vivo suppressor T cells which inhibited the activity of anti-tumor cytotoxic T cells.
通过伴刀豆球蛋白A亲和层析和凝胶过滤回收去污剂溶解的糖蛋白，从12型腺病毒（Ad12）仓鼠肿瘤细胞的膜组分中分离出两种类型的糖蛋白。其中一种糖蛋白由分子量为130,000道尔顿（130K）、pI值为4.7 - 5.1的多肽组成，另一种由分子量为18,500道尔顿（18.5K）、pI值为6.3 - 6.6的多肽组成。这些糖蛋白在免疫学上是不同的。18.5K糖蛋白在体内诱导对肿瘤生长的抗性和抗肿瘤细胞毒性T细胞，而130K糖蛋白在体内诱导抑制抗肿瘤细胞毒性T细胞活性的抑制性T细胞。
