Kamm A R, Grimes W J
Proc Natl Acad Sci U S A. 1978 Dec;75(12):5912-6. doi: 10.1073/pnas.75.12.5912.
This work describes the detection, isolation, and partial characterization of a BALB/c mouse fibroblast cell surface antigen. This antigen migrates as a polypeptide of approximately 100,000 daltons in a discontinuous sodium dodecyl sulfate/polyacrylamide gel electrophoresis system, can be labeled by either lactoperoxidase-catalyzed cell surface 125I iodination or metabolic incorporation of [3H]glucosamine, and can be isolated by concanavalin A affinity chromatography. This cell surface glycoprotein is antigenic in BALB/c mice and has been correlated with the rejection of immunogenic tumor cells. Also, antiserum specific for Moloney leukemia virus precipitates the 100,000-dalton cell surface protein from viral and immunogenic spontaneous transformants. This virus-related antigen comigrates on sodium dodecyl sulfate gels with the major iodinated cell surface protein of these transformants. Rabbit antiserum to the purified antigen demonstrates a marked preference for the surfaces of immunogenic tumor cells as compared with normal cells and nonimmunogenic tumor cells.
这项研究描述了BALB/c小鼠成纤维细胞表面抗原的检测、分离及部分特性。该抗原在不连续的十二烷基硫酸钠/聚丙烯酰胺凝胶电泳系统中以大约100,000道尔顿的多肽形式迁移，可通过乳过氧化物酶催化的细胞表面125I碘化或[3H]葡糖胺的代谢掺入进行标记，并可通过伴刀豆球蛋白A亲和层析进行分离。这种细胞表面糖蛋白在BALB/c小鼠中具有抗原性，并且与免疫原性肿瘤细胞的排斥反应相关。此外，针对莫洛尼白血病病毒的特异性抗血清可沉淀来自病毒和免疫原性自发转化体的100,000道尔顿细胞表面蛋白。这种病毒相关抗原在十二烷基硫酸钠凝胶上与这些转化体的主要碘化细胞表面蛋白共迁移。与正常细胞和非免疫原性肿瘤细胞相比，针对纯化抗原的兔抗血清对免疫原性肿瘤细胞表面表现出明显的偏好。
