Julesz J, Lis M, Gatkowska J, Mizsei I, Genest J
Acta Med Hung. 1984;41(1):35-41.
Using a primary cell culture of rat anterior pituitary and corticotropin (ACTH) RIA, the effects of various steroids have been investigated. Dexamethasone inhibited the ACTH release stimulated by alpha-melanotropin and Pitressin (posterior pituitary extract), but did not influence the non-stimulated release. The effect of all the investigated steroids was significant; aldosterone exhibited a more marked inhibition than spironolactone. Simultaneous administration of aldosterone and spironolactone resulted predominantly in the manifestation of spironolactone action. A new effect of spironolactone has been demonstrated in vitro; the evaluation of its pharmacological importance requires studies in vivo.
利用大鼠垂体前叶原代细胞培养和促肾上腺皮质激素（ACTH）放射免疫分析法，研究了各种类固醇的作用。地塞米松抑制α-促黑素和垂体后叶素（垂体后叶提取物）刺激的ACTH释放，但不影响非刺激状态下的释放。所有研究的类固醇的作用均显著；醛固酮的抑制作用比螺内酯更明显。同时给予醛固酮和螺内酯主要表现出螺内酯的作用。已在体外证明了螺内酯的一种新作用；对其药理学重要性的评估需要进行体内研究。
