Cimini V
Institute of Human Anatomy, Medical School of Federico II University of Naples, Italy.
Exp Cell Res. 1996 Nov 1;228(2):212-5. doi: 10.1006/excr.1996.0319.
Double labeling of dispersed anterior pituitary cells revealed the coexistence of galanin immunoreactivity with adrenocorticotropic hormone (ACTH) as well as prolactin. However, only laser confocal microscopy showed three different areas of immunoreactivity within the corticotroph cytoplasm, two of them for ACTH and galanin separately and the third containing both immunoreactivities. To determine a possible relation between ACTH cells and galanin, 4-day cultures of anterior pituitary cells from female rats were examined by cell blot assay, and they showed ACTH release inhibition by 10(-6)M galanin. Furthermore, after 17 beta-estradiol treatment to maximize lactotroph galanin release in vitro, the cell blotting-assessed secretory level of corticotroph cells was very similar to that of cells in the presence of 10(-6)M galanin. In fact, immunoneutralization with galanin antiserum quenched the inhibitory effect of galanin on ACTH secretion. Our study suggests that locally produced galanin can modulate corticotropin release.
对分散的垂体前叶细胞进行双重标记显示，甘丙肽免疫反应性与促肾上腺皮质激素（ACTH）以及催乳素共存。然而，只有激光共聚焦显微镜显示促肾上腺皮质激素细胞胞质内存在三个不同的免疫反应区域，其中两个分别针对ACTH和甘丙肽，第三个区域同时含有这两种免疫反应性物质。为了确定ACTH细胞与甘丙肽之间可能存在的关系，采用细胞印迹分析法对雌性大鼠垂体前叶细胞进行了4天的培养，结果显示10(-6)M甘丙肽可抑制ACTH释放。此外，在体外经17β-雌二醇处理以使泌乳细胞的甘丙肽释放最大化后，细胞印迹法评估的促肾上腺皮质激素细胞分泌水平与存在10(-6)M甘丙肽时的细胞分泌水平非常相似。事实上，用甘丙肽抗血清进行免疫中和可消除甘丙肽对ACTH分泌的抑制作用。我们的研究表明，局部产生的甘丙肽可调节促肾上腺皮质激素的释放。
