Sancar G B, Sancar A, Rupp W D
Nucleic Acids Res. 1984 Jun 11;12(11):4593-608. doi: 10.1093/nar/12.11.4593.
We have determined the sequence of a 2400 bp region of E. coli chromosomal DNA containing the uvrC gene. The coding region of uvrc is 2267 bp in length, encodes a polypeptide with a calculated molecular weight of 66,038 daltons, and is preceded by a typical E. coli ribosome binding site. By constructing deletion derivatives we have established that a uvrC promoter lies within the 113 bp region preceding the translational start of uvrC. The codon usage in uvrC is strongly biased in favor of codons used infrequently in E. coli, which may contribute to the relatively low intracellular concentration of uvrC protein.
我们已经确定了大肠杆菌染色体DNA中包含uvrC基因的一个2400 bp区域的序列。uvrC的编码区长度为2267 bp，编码一种计算分子量为66,038道尔顿的多肽，并且其前面有一个典型的大肠杆菌核糖体结合位点。通过构建缺失衍生物，我们确定uvrC启动子位于uvrC翻译起始位点之前的113 bp区域内。uvrC中的密码子使用强烈偏向于在大肠杆菌中很少使用的密码子，这可能导致uvrC蛋白在细胞内的浓度相对较低。
