Albrecht P, van Steenis G, van Wezel A L, Salk J
Rev Infect Dis. 1984 May-Jun;6 Suppl 2:S540-4. doi: 10.1093/clinids/6.supplement_2.s540.
Recently the Forum for Advancement of Immunization Research sponsored a Collaborative Study on Poliovirus Antibody Titration. Twenty laboratories from 12 countries participated. There were considerable differences in detail of test performance and test results among laboratories. The sensitivity of the tests varied over a 10-fold range (geometric mean titer from 21 to 288). The average coefficient of variation ranged from 4.5% to 13.5%. Tests performed at the Food and Drug Administration indicated that Hep-2 cells were more suitable than Vero cells for poliovirus titration by cytopathic effect end point or plaque assay. However, the cell lines were equally suitable for polio antibody titration by neutralization of cytopathic effect. A high degree of sensitivity and optimal reproducibility of antibody assay were observed in tests utilizing a medium to low dose of virus and serum-virus incubation overnight at 36 C. On the basis of current experience, a protocol is proposed for a test that provides high sensitivity and reproducibility and is practical for large-scale determinations of poliovirus antibody.
最近，免疫研究促进论坛发起了一项关于脊髓灰质炎病毒抗体滴定的合作研究。来自12个国家的20个实验室参与其中。各实验室在检测操作细节和检测结果方面存在相当大的差异。检测的灵敏度在10倍范围内变化（几何平均滴度从21到288）。平均变异系数在4.5%至13.5%之间。美国食品药品监督管理局进行的检测表明，对于通过细胞病变效应终点或空斑试验进行脊髓灰质炎病毒滴定，Hep-2细胞比Vero细胞更合适。然而，通过中和细胞病变效应进行脊髓灰质炎抗体滴定，这两种细胞系同样合适。在使用中低剂量病毒且血清与病毒在36℃下孵育过夜的检测中，观察到抗体检测具有高度的灵敏度和最佳的可重复性。基于目前的经验，提出了一种检测方案，该方案具有高灵敏度和可重复性，并且对于大规模测定脊髓灰质炎病毒抗体是实用的。
