Grisoni M, Meneguzzi G, de Lapeyrière O, Binétruy B, Rassoulzadegan M, Cuzin F
Virology. 1984 Jun;135(2):406-16. doi: 10.1016/0042-6822(84)90196-x.
Unlike cell lines transformed with Polyomaviruses, transformants derived by focus formation or colony formation in agarose medium after transfer into rat fibroblast cells (FR3T3 line) of Bovine Papilloma Type 1 (BPV1) DNA were consistently observed to grow poorly in suspension and to remain highly serum dependent for growth in culture. These cells did not produce detectable amounts of plasminogen activator, and kept the flat morphology and organized cytoskeleton characteristic of the normal fibroblast. However, they induced in syngeneic animals the development of tumors with a greater invasive potential than tumors induced by Polyomaviruses. By contrast with the original transformants, cells recovered from the tumors grew efficiently in suspension and produced high levels of plasminogen activator. They still had, however, extended cytoskeletal structures and remained completely dependent on high serum concentrations for growth in culture. The stepwise transformation process induced by BPV1 thus appears strikingly different from that previously observed with polyoma and SV40 viruses. The observed changes in transformation phenotype between transformed line and tumor cells do not correlate with any important modification of the number of autonomous copies of the viral genome, nor with any rearrangement of viral sequences detectable at the level of the blot analysis.
与用多瘤病毒转化的细胞系不同，将1型牛乳头瘤病毒（BPV1）DNA转入大鼠成纤维细胞（FR3T3系）后，通过在琼脂糖培养基中形成集落或灶性形成而获得的转化体，始终表现出在悬浮培养中生长不佳，并且在培养过程中对血清高度依赖。这些细胞未产生可检测量的纤溶酶原激活物，保持了正常成纤维细胞的扁平形态和有组织的细胞骨架特征。然而，它们在同基因动物中诱导产生的肿瘤比多瘤病毒诱导的肿瘤具有更大的侵袭潜力。与原始转化体相比，从肿瘤中回收的细胞在悬浮培养中生长良好，并产生高水平的纤溶酶原激活物。然而，它们仍然具有延伸的细胞骨架结构，并且在培养过程中完全依赖高血清浓度生长。因此，BPV1诱导的逐步转化过程与先前观察到的多瘤病毒和SV40病毒的转化过程明显不同。在转化细胞系和肿瘤细胞之间观察到的转化表型变化，与病毒基因组自主复制数目的任何重要改变无关，也与在印迹分析水平上可检测到的病毒序列的任何重排无关。
