Hussain A, Mohanty S B
Am J Vet Res. 1984 Jun;45(6):1219-21.
An antibody-complement-mediated cytotoxicity assay was employed to determine whether susceptible host cells infected with bovine parainfluenza-3 virus could be destroyed by the humoral immune mechanism. The cytotoxicity assay yielded 21.5% to 76.9% specific release of 51Cr with sera collected from calves 28 days after inoculation with the virus. A multiplicity of infection of 5, a postinfection period of 2 days, an antibody dilution of 1:10, and an incubation period of 12 hours with antibody and complement were found to be optimal for this assay.
采用抗体-补体介导的细胞毒性试验来确定感染牛副流感3型病毒的易感宿主细胞是否能被体液免疫机制破坏。用接种病毒28天后从小牛采集的血清进行细胞毒性试验，51Cr的特异性释放率为21.5%至76.9%。发现感染复数为5、感染后2天、抗体稀释度为1:10以及抗体与补体孵育12小时对该试验最为适宜。
