Ghram A, Reddy P G, Morrill J L, Blecha F, Minocha H C
Department of Laboratory Medicine, Kansas State University, Manhattan 66506.
Can J Vet Res. 1989 Jan;53(1):62-7.
Calves infected with bovine herpesvirus-1 (BHV-1) or both BHV-1 and parainfluenza-3 virus (PIV-3) developed clinical signs including fever, cough, and nasal and ocular discharges. Animals infected with both viruses appeared more depressed and showed higher rectal temperature, while calves inoculated with PIV-3 alone had a very mild clinical disease. Both BHV-1 and PIV-3 were recovered from nasal secretions up to six to eight days postinoculation. However, the virus titers were lower in calves with mixed infection. An increase in serum antibodies to both BHV-1 and PIV-3 was detected by serum neutralization and enzyme-linked immunosorbent assay. Antibody responses were delayed and significantly lower in calves given mixed infection than in calves infected with a single virus. Interleukin-2 activity in cultures of lymphocytes from BHV-1 and BHV-1 plus PIV-3 infected calves was higher compared to control calves.
感染牛疱疹病毒1型（BHV - 1）或同时感染BHV - 1和副流感病毒3型（PIV - 3）的犊牛出现了包括发热、咳嗽、鼻眼分泌物增多等临床症状。感染两种病毒的动物显得更加萎靡，直肠温度更高，而单独接种PIV - 3的犊牛临床疾病症状非常轻微。接种后六至八天内，在鼻分泌物中均可检测到BHV - 1和PIV - 3。然而，混合感染的犊牛病毒滴度较低。通过血清中和试验和酶联免疫吸附测定法检测到血清中针对BHV - 1和PIV - 3的抗体均有所增加。混合感染的犊牛抗体反应延迟，且明显低于单一病毒感染的犊牛。与对照犊牛相比，感染BHV - 1以及感染BHV - 1加PIV - 3的犊牛淋巴细胞培养物中的白细胞介素-2活性更高。
