Caligaris-Cappio F, Janossy G, Campana D, Chilosi M, Bergui L, Foà R, Delia D, Giubellino M C, Preda P, Gobbi M
Leuk Res. 1984;8(4):567-78. doi: 10.1016/0145-2126(84)90005-5.
The tumor promoting agent TPA (phorbol ester; 1.6 X 10(-8)M) was used to induce the differentiation in vitro of B-chronic lymphocytic leukemia (B-CLL) cells from 14 untreated patients. The uninduced phenotype was SIg+, Mrbc+, RFT-1+, RFA-4-, FMC7-. After 72 h incubation with TPA, B-CLL cells became RFA-4+, FMC7+ and lost the capability of Mrbc rosetting. Large proportions of the "induced" cells also showed morphological and ultrastructural changes, such as undulating membranes and bleblike protusions and became strongly positive for tartrate resistant acid phosphatase (TRAP+) and also contained cytoplasmic immunoglobulins. These features are very similar to the features of hairy cell leukemia (HCL). These observations confirm previous clinical findings that B-CLL and HCL are related disorders of the B lineage. The development of "hairy" features in induced B-CLL and in HCL seems to be a malignancy-associated feature because the Mrbc+ normal B cells (B-CLL-equivalent cells) isolated from tonsil also develop TRAP positivity but no membrane aberrations.
肿瘤促进剂TPA（佛波酯；1.6×10⁻⁸M）被用于诱导14例未经治疗患者的B慢性淋巴细胞白血病（B-CLL）细胞进行体外分化。未诱导的表型为SIg⁺、Mrbc⁺、RFT-1⁺、RFA-4⁻、FMC7⁻。用TPA孵育72小时后，B-CLL细胞变为RFA-4⁺、FMC7⁺，并丧失了Mrbc玫瑰花结形成能力。大部分“诱导”细胞还表现出形态学和超微结构变化，如起伏的细胞膜和泡状突起，并对耐酒石酸酸性磷酸酶呈强阳性（TRAP⁺），且含有细胞质免疫球蛋白。这些特征与毛细胞白血病（HCL）的特征非常相似。这些观察结果证实了先前的临床发现，即B-CLL和HCL是B系相关疾病。诱导的B-CLL和HCL中“毛状”特征的出现似乎是一种与恶性肿瘤相关的特征，因为从扁桃体分离的Mrbc⁺正常B细胞（B-CLL等效细胞）也出现TRAP阳性，但没有膜异常。
