Visser L, Poppema S
Department of Laboratory Medicine, Cross Cancer Institute, Edmonton, Alberta, Canada.
Br J Haematol. 1990 Jul;75(3):359-65. doi: 10.1111/j.1365-2141.1990.tb04349.x.
To investigate the relationship between normal B-cells, B-cell chronic lymphocytic leukaemia (B-CLL) cells and hairy cell leukaemia (HCL) cells the three cell types were incubated with phorbol myristic acetate (PMA). The parameters studied were morphology, immunophenotype and tartrate resistant acid phosphatase (TRAP). PMA stimulation of B-cells induced morphological changes as well as CD5, CD11c, B-ly 7 and TRAP positivity. The cells formed small aggregates, the cell membranes were ruffled and frequently hairy and a small number of cells became plastic adherent and developed dendritic structure. CD5 and CD11c appeared on day 2, imitating a B-CLL phenotype. On day 3 and 4 a decrease of CD5, an increase of CD11c and the appearance of B-ly 7 could be seen mimicking an HCL phenotype. The changes in B-CLL and HCL upon PMA stimulation were mainly morphological: the B-CLL cells became ruffled and aggregated strongly, while the HCL cells developed dendritic features and became adherent. The immunophenotype of the PMA stimulated HCL cells did not change. The B-CLL cells remained CD5 positive and did not become B-ly 7 positive. The findings indicate that although PMA stimulation of normal peripheral blood cells results in CLL- as well as HCL-like phenotypes, similar stimulation of B-CLL cells does not result in an HCL-like phenotype.
为了研究正常B细胞、B细胞慢性淋巴细胞白血病（B-CLL）细胞和毛细胞白血病（HCL）细胞之间的关系，将这三种细胞类型与佛波醇肉豆蔻酸酯（PMA）一起孵育。所研究的参数包括形态学、免疫表型和耐酒石酸酸性磷酸酶（TRAP）。PMA刺激B细胞会诱导形态学变化以及CD5、CD11c、B-ly 7和TRAP阳性。细胞形成小聚集体，细胞膜出现褶皱且常有毛刺，少数细胞变得可塑贴壁并形成树突状结构。CD5和CD11c在第2天出现，模仿B-CLL表型。在第3天和第4天，可以看到CD5减少、CD11c增加以及B-ly 7出现，模仿HCL表型。PMA刺激后B-CLL和HCL的变化主要是形态学上的：B-CLL细胞变得有褶皱并强烈聚集，而HCL细胞则形成树突状特征并变得贴壁。PMA刺激的HCL细胞的免疫表型没有变化。B-CLL细胞保持CD5阳性，没有变成B-ly 7阳性。研究结果表明，尽管PMA刺激正常外周血细胞会导致CLL样和HCL样表型，但类似地刺激B-CLL细胞并不会导致HCL样表型。
