Caligaris-Cappio F, Pizzolo G, Chilosi M, Bergui L, Semenzato G, Tesio L, Morittu L, Malavasi F, Gobbi M, Schwarting R
Blood. 1985 Nov;66(5):1035-42.
We have investigated the relationship between chronic lymphocytic leukemia (CLL), hairy cell leukemia (HCL), and different normal B cell subsets: Mrbc+, T1+ and slgM+ tonsil cells; germinal center; mantle zone; and peripheral blood B lymphocytes. Both malignant and normal cells were incubated in vitro with the phorbol ester 12-O-tetradecanoyl-phorbol-13-acetate (TPA) for 72 hours and the morphology, cytochemical profile, and surface markers were evaluated. The results show that CLL cells TPA-induced become indistinguishable from HCL by four independent criteria: the morphology; the cytoplasmic tartrate resistant acid phosphatase (TRAP) enzyme activity; the membrane positivity with anti-Leu M5 (SHCL3); and anti-Tac monoclonal antibodies which, in the uninduced state, react only with HCL. The features of TRAP and Tac positivity are also expressed (though in variable degree) by different normal B cell populations activated with TPA or pokeweed mitogen (PWM). It is concluded that HCL might represent an aberrantly activated variant of CLL (or of a CLL-related disorder).
我们研究了慢性淋巴细胞白血病（CLL）、毛细胞白血病（HCL）与不同正常B细胞亚群之间的关系：Mrbc +、T1 +和表面免疫球蛋白M（slgM）+扁桃体细胞；生发中心；套区；以及外周血B淋巴细胞。将恶性细胞和正常细胞在体外与佛波酯12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯（TPA）孵育72小时，并对形态、细胞化学特征和表面标志物进行评估。结果表明，经TPA诱导的CLL细胞在四个独立标准方面变得与HCL难以区分：形态；细胞质抗酒石酸酸性磷酸酶（TRAP）酶活性；抗Leu M5（SHCL3）的膜阳性；以及抗Tac单克隆抗体，在未诱导状态下，抗Tac单克隆抗体仅与HCL反应。TRAP阳性和Tac阳性特征也由用TPA或商陆有丝分裂原（PWM）激活的不同正常B细胞群体表达（尽管程度不同）。得出的结论是，HCL可能代表CLL（或与CLL相关的疾病）的异常激活变体。
