Microtechniques for flow cytometric analysis of aqueous humor lymphocytes.
作者信息
Freeman W R, Char D H, Garavoy M R, Lyon H
出版信息
J Immunol Methods. 1984 Oct 26;73(2):447-55. doi: 10.1016/0022-1759(84)90420-4.
PMID:6333465
Abstract
Flow cytometric analysis of monoclonal antibody labelled lymphocytes is an important tool in understanding the immune response. Microtechniques were developed to analyze lymphocytes in small amounts of ocular fluids. Optimal labelling volumes and concentrations for Leu 2a, Leu 3a, Leu 4 and Leu M3 antibodies were determined. The ability to label and reproducibly analyze cell samples with as few as 1000 cells is demonstrated.
摘要
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