Wang X C, Norose K, Yano A, Ohta K, Segawa K
Department of Ophthalmology, Shinshu University School of Medicine, Matsumoto, Japan.
Curr Eye Res. 1995 Jun;14(6):425-33. doi: 10.3109/02713689509003752.
To investigate the immunopathologic role of lymphocytes in aqueous humor of patients with uveitis, we assessed activated T lymphocyte subsets stained with fluorescein isothiocyanate-labeled and phycoerythrin-labeled monoclonal antibodies in aqueous humor and in peripheral blood, using two-color flow cytometry. We divided our 25 patients into those with endogenous or exogenous uveitis. The percentages of activated T lymphocytes in uveitic aqueous humor significantly exceeded those in peripheral blood lymphocytes from patients with either disease form. CD4+HLA-DR+ lymphocytes were predominant in endogenous uveitic aqueous humor, whereas CD8+HLA-DR+ lymphocytes predominated in exogenous uveitic aqueous humor in the early stage of the disease. The percentages of CD4+HLA-DR+ lymphocytes tended to increase while those of CD8+HLA-DR+ cells tended to decrease in aqueous humor from patients in the late phase of exogenous uveitis. Our results may contribute to increasing diagnostic accuracy in patients with uveitis and to developing more rational methods of treatment.
为研究淋巴细胞在葡萄膜炎患者房水中的免疫病理作用，我们采用双色流式细胞术，评估了用异硫氰酸荧光素标记和藻红蛋白标记的单克隆抗体染色的房水及外周血中活化的T淋巴细胞亚群。我们将25例患者分为内源性或外源性葡萄膜炎患者。两种疾病形式患者的葡萄膜炎房水中活化T淋巴细胞的百分比显著超过外周血淋巴细胞中的百分比。内源性葡萄膜炎房水中以CD4+HLA-DR+淋巴细胞为主，而在疾病早期外源性葡萄膜炎房水中以CD8+HLA-DR+淋巴细胞为主。在外源性葡萄膜炎晚期患者的房水中，CD4+HLA-DR+淋巴细胞的百分比趋于增加，而CD8+HLA-DR+细胞的百分比趋于下降。我们的结果可能有助于提高葡萄膜炎患者的诊断准确性，并有助于开发更合理的治疗方法。
