Beresford J N, Gallagher J A, Poser J W, Russell R G
Metab Bone Dis Relat Res. 1984;5(5):229-34. doi: 10.1016/0221-8747(84)90064-x.
Cells have been cultured from human bone that possess several characteristics of osteoblasts, including the capacity to produce osteocalcin (bone Gla protein). In these cultures the production of osteocalcin is dependent on 1,25(OH)2D3 but is not affected by 24,25(OH)2D3 either alone or in combination with 1,25(OH)2D3. Two glucocorticoids, prednisolone and deflazacort, reverse the stimulation of osteocalcin synthesis by 1,25(OH)2D3 in a dose-dependent manner (10(-9) - 10(-6)M). Parathyroid hormone also inhibits osteocalcin production in a dose-dependent fashion (0.2-5 IU/ml). These results demonstrate that human bone cell cultures may be of considerable value in investigating the hormonal and pharmacologic regulation of the production of osteocalcin and other bone proteins in vitro.
已从人骨中培养出具有成骨细胞若干特性的细胞，包括产生骨钙素（骨γ-羧基谷氨酸蛋白）的能力。在这些培养物中，骨钙素的产生依赖于1,25(OH)₂D₃，但不受单独的24,25(OH)₂D₃或与1,25(OH)₂D₃联合使用的影响。两种糖皮质激素，泼尼松龙和地夫可特，以剂量依赖方式（10⁻⁹ - 10⁻⁶M）逆转1,25(OH)₂D₃对骨钙素合成的刺激作用。甲状旁腺激素也以剂量依赖方式（0.2 - 5 IU/ml）抑制骨钙素的产生。这些结果表明，人骨细胞培养物在体外研究骨钙素和其他骨蛋白产生的激素和药理调节方面可能具有相当大的价值。
