人白细胞介素2的氨基酸序列与翻译后修饰
Amino acid sequence and post-translational modification of human interleukin 2.
作者信息
Robb R J, Kutny R M, Panico M, Morris H R, Chowdhry V
出版信息
Proc Natl Acad Sci U S A. 1984 Oct;81(20):6486-90. doi: 10.1073/pnas.81.20.6486.
Abstract
Human interleukin 2 was separated into multiple molecular forms by selective immunoaffinity chromatography and chromatofocusing. For the most part, this heterogeneity was attributed to variations in glycosylation of the threonine residue in position 3 of the polypeptide chain. The various molecular forms of interleukin 2 had nearly identical specific activities in the in vitro proliferation assay, indicating that the glycosylation had no significant effect on this response. The entire primary sequence of interleukin 2, including the location of the intramolecular disulfide bridge, was determined by a combination of peptide mapping and protein sequencing. This information should aid in the determination of the active site(s) of the molecule.
摘要
通过选择性免疫亲和层析和色谱聚焦法，人白细胞介素2被分离成多种分子形式。在很大程度上，这种异质性归因于多肽链第3位苏氨酸残基糖基化的差异。白细胞介素2的各种分子形式在体外增殖试验中具有几乎相同的比活性，这表明糖基化对该反应没有显著影响。通过肽图分析和蛋白质测序相结合的方法，确定了白细胞介素2的完整一级序列，包括分子内二硫键的位置。这些信息应有助于确定该分子的活性位点。
相似文献
1
Amino acid sequence and post-translational modification of human interleukin 2.人白细胞介素2的氨基酸序列与翻译后修饰
Proc Natl Acad Sci U S A. 1984 Oct;81(20):6486-90. doi: 10.1073/pnas.81.20.6486.
2
Posttranslational modification of human T-cell growth factor.人T细胞生长因子的翻译后修饰
Biochem Biophys Res Commun. 1983 Nov 15;116(3):1049-55. doi: 10.1016/s0006-291x(83)80248-4.
3
Peptide, disulfide, and glycosylation mapping of recombinant human thrombopoietin from ser1 to Arg246.重组人血小板生成素从Ser1至Arg246的肽段、二硫键及糖基化图谱分析
Biochemistry. 1996 Nov 26;35(47):14849-61. doi: 10.1021/bi961075b.
4
Structure-function relationships for the IL-2 receptor system. V. Structure-activity analysis of modified and truncated forms of the Tac receptor protein: site-specific mutagenesis of cysteine residues.白细胞介素-2受体系统的结构-功能关系。V. Tac受体蛋白修饰和截短形式的结构-活性分析：半胱氨酸残基的位点特异性诱变
J Immunol. 1988 Apr 1;140(7):2249-59.
5
Heterogeneity of the covalent structure of the blue copper protein umecyanin from horseradish roots.辣根根中蓝色铜蛋白乌美花青素共价结构的异质性。
Protein Sci. 1995 Feb;4(2):209-27. doi: 10.1002/pro.5560040208.
6
Glycosylation pattern and disulfide assignments of recombinant human differentiation-stimulating factor.
Arch Biochem Biophys. 1993 May;302(2):484-9. doi: 10.1006/abbi.1993.1243.
7
Modification and secretion of human interleukin 2 produced in insect cells by a baculovirus expression vector.杆状病毒表达载体在昆虫细胞中产生的人白细胞介素2的修饰与分泌
Proc Natl Acad Sci U S A. 1985 Dec;82(24):8404-8. doi: 10.1073/pnas.82.24.8404.
8
Alternate amino terminal processing of surfactant protein A results in cysteinyl isoforms required for multimer formation.表面活性蛋白A的交替氨基末端加工产生多聚体形成所需的半胱氨酸亚型。
Biochemistry. 1997 Jun 10;36(23):7018-25. doi: 10.1021/bi970100q.
9
Disulfide structure of the heparin binding domain in vascular endothelial growth factor: characterization of posttranslational modifications in VEGF.血管内皮生长因子中肝素结合域的二硫键结构：血管内皮生长因子翻译后修饰的特征
Arch Biochem Biophys. 1997 Aug 1;344(1):103-13. doi: 10.1006/abbi.1997.0145.
10
Chromogranin A from bovine adrenal medulla: molecular characterization of glycosylations, phosphorylations, and sequence heterogeneities by mass spectrometry.来自牛肾上腺髓质的嗜铬粒蛋白A：通过质谱对糖基化、磷酸化和序列异质性的分子表征
Anal Biochem. 1999 Oct 1;274(1):69-80. doi: 10.1006/abio.1999.4244.
引用本文的文献
1
Implications of glycosylation for the development of selected cytokines and their derivatives for medical use.糖基化对选定细胞因子及其衍生物在医学用途方面的发展的影响。
Biotechnol Adv. 2024 Dec;77:108467. doi: 10.1016/j.biotechadv.2024.108467. Epub 2024 Oct 22.
2
Next generation of multispecific antibody engineering.下一代多特异性抗体工程。
Antib Ther. 2023 Dec 8;7(1):37-52. doi: 10.1093/abt/tbad027. eCollection 2024 Jan.
3
The T-Cell Growth Factor Interleukin-2, Which Is Occasionally Targeted by Autoantibodies, Qualifies as Drug for the Treatment of Allergy, Autoimmunity, and Cancer: Collegium Internationale Allergologicum (CIA) Update 2024.T细胞生长因子白细胞介素-2偶尔会成为自身抗体的靶点，有资格作为治疗过敏、自身免疫和癌症的药物：国际变态反应学会（CIA）2024年更新。
Int Arch Allergy Immunol. 2024;185(3):286-300. doi: 10.1159/000533677. Epub 2023 Dec 12.
4
O-glycosylation and its role in therapeutic proteins.O-糖基化及其在治疗性蛋白质中的作用。
Biosci Rep. 2022 Oct 28;42(10). doi: 10.1042/BSR20220094.
5
Improving Immunotherapy Through Glycodesign.通过糖基设计提高免疫疗法。
Front Immunol. 2018 Nov 2;9:2485. doi: 10.3389/fimmu.2018.02485. eCollection 2018.
6
Insufficient (sub-native) helix content in soluble/solid aggregates of recombinant and engineered forms of IL-2 throws light on how aggregated IL-2 is biologically active.重组和工程形式的 IL-2 的可溶性/固态聚集物中存在（亚天然）螺旋结构不足，这揭示了聚集的 IL-2 如何具有生物活性。
Protein J. 2012 Oct;31(7):529-43. doi: 10.1007/s10930-012-9429-2.
7
Prediction of mucin-type O-glycosylation sites by a two-staged strategy.通过两阶段策略预测粘蛋白型 O-糖基化位点。
Mol Divers. 2011 May;15(2):427-33. doi: 10.1007/s11030-010-9240-y. Epub 2010 Jul 22.
8
Glycoproteomics: past, present and future.糖蛋白质组学：过去、现在与未来。
FEBS Lett. 2009 Jun 5;583(11):1728-35. doi: 10.1016/j.febslet.2009.03.049. Epub 2009 Mar 27.
9
The first alpha helix of interleukin (IL)-2 folds as a homotetramer, acts as an agonist of the IL-2 receptor beta chain, and induces lymphokine-activated killer cells.白细胞介素（IL）-2的第一个α螺旋折叠成同四聚体，作为IL-2受体β链的激动剂，并诱导淋巴因子激活的杀伤细胞。
J Exp Med. 2000 Feb 7;191(3):529-40. doi: 10.1084/jem.191.3.529.
10
NetOglyc: prediction of mucin type O-glycosylation sites based on sequence context and surface accessibility.NetOglyc：基于序列上下文和表面可及性预测粘蛋白型O-糖基化位点
Glycoconj J. 1998 Feb;15(2):115-30. doi: 10.1023/a:1006960004440.
本文引用的文献
1
T-cell growth factor.T细胞生长因子。
Immunol Rev. 1980;51:337-57. doi: 10.1111/j.1600-065x.1980.tb00327.x.
2
Heterogeneity of human T-cell growth factor(s) due to variable glycosylation.由于糖基化可变导致的人T细胞生长因子的异质性。
Mol Immunol. 1981 Dec;18(12):1087-94. doi: 10.1016/0161-5890(81)90024-9.
3
Posttranslational modification of human T-cell growth factor.人T细胞生长因子的翻译后修饰
Biochem Biophys Res Commun. 1983 Nov 15;116(3):1049-55. doi: 10.1016/s0006-291x(83)80248-4.
4
Purification and partial sequence analysis of human T-cell growth factor.人T细胞生长因子的纯化及部分序列分析
Proc Natl Acad Sci U S A. 1983 Oct;80(19):5990-4. doi: 10.1073/pnas.80.19.5990.
5
A rapid, large scale purification procedure for gibbon interleukin 2.一种用于长臂猿白细胞介素2的快速大规模纯化方法。
J Immunol. 1983 Aug;131(2):810-5.
6
Analysis of T cell function in autoimmune murine strains. Defects in production and responsiveness to interleukin 2.自身免疫性小鼠品系中T细胞功能分析。白细胞介素2产生及反应性缺陷。
J Exp Med. 1981 Sep 1;154(3):791-808. doi: 10.1084/jem.154.3.791.
7
Structure and expression of a cloned cDNA for human interleukin-2.人白细胞介素-2克隆cDNA的结构与表达
Nature. 1983;302(5906):305-10. doi: 10.1038/302305a0.
8
Molecular cloning of human interleukin 2 cDNA and its expression in E. coli.人白细胞介素2 cDNA 的分子克隆及其在大肠杆菌中的表达。
Nucleic Acids Res. 1983 Jul 11;11(13):4307-23. doi: 10.1093/nar/11.13.4307.
9
Comparative aspects of glycoprotein structure.糖蛋白结构的比较方面。
Annu Rev Biochem. 1976;45:217-37. doi: 10.1146/annurev.bi.45.070176.001245.
Zotero 插件