人白细胞介素-2克隆cDNA的结构与表达
Structure and expression of a cloned cDNA for human interleukin-2.
作者信息
Taniguchi T, Matsui H, Fujita T, Takaoka C, Kashima N, Yoshimoto R, Hamuro J
出版信息
Nature. 1983;302(5906):305-10. doi: 10.1038/302305a0.
DOI:10.1038/302305a0
PMID:6403867
Abstract
A cDNA coding for human interleukin-2 (IL-2) has been cloned from a cDNA library prepared from partially purified IL-2 mRNA. The DNA sequence codes for a polypeptide which consists of 153 amino acids including a putative signal sequence. A biologically active polypeptide, characteristic of human IL-2, was produced when the cDNA was fused to a simian virus 40 promoter sequence and used to transfect cultured monkey COS cells.
摘要
已从由部分纯化的白细胞介素-2（IL-2）信使核糖核酸制备的互补脱氧核糖核酸（cDNA）文库中克隆出编码人白细胞介素-2的cDNA。该DNA序列编码一种由153个氨基酸组成的多肽，其中包括一个推定的信号序列。当该cDNA与猿猴病毒40启动子序列融合并用于转染培养的猴COS细胞时，产生了一种具有人IL-2特征的生物活性多肽。
Zotero 插件