Beiske K, Ruud E, Marton P F, Godal T
Scand J Immunol. 1984 Sep;20(3):199-208. doi: 10.1111/j.1365-3083.1984.tb00993.x.
Cells from eight selected cases of human non-Hodgkin lymphomas of various histological types (lymphocytic, centrocytic, centrocytic/centroblastic, and immunocytomas) were stimulated in vitro with 12-O-tetradecanoyl-phorbol-13-acetate (TPA) and anti-immunoglobulins (anti-Ig) against the surface immunoglobulin (sIg) on the tumour cells. Six of these cases responded by intracellular Ig accumulation as measured by flow cytofluorometry and direct phenotypical change into immunoblasts/plasmablasts as detected by light microscopic immunocytochemistry. However, the response to TPA alone varied considerably from case to case. These findings suggest that many, if not all, B-cell subsets have the capacity to develop directly into Ig-synthesizing cells (immunoblasts and plasmablasts). However, conditions for eliciting such events may vary, depending on the phenotypical properties and differentiation stage.
选取了8例不同组织学类型（淋巴细胞型、中心细胞型、中心细胞/中心母细胞型和免疫细胞瘤）的人类非霍奇金淋巴瘤病例，其细胞在体外分别用12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯（TPA）和针对肿瘤细胞表面免疫球蛋白（sIg）的抗免疫球蛋白（抗Ig）进行刺激。通过流式细胞荧光测定法测定，其中6例出现细胞内Ig积累，通过光学显微镜免疫细胞化学检测，出现直接表型转变为免疫母细胞/成浆细胞。然而，单独对TPA的反应在不同病例间差异很大。这些发现表明，许多（即便不是全部）B细胞亚群都有直接发育为Ig合成细胞（免疫母细胞和成浆细胞）的能力。然而，引发此类事件的条件可能因表型特性和分化阶段而异。
