Godal T, Henriksen A, Ruud E, Michaelsen T
Scand J Immunol. 1982 Mar;15(3):267-74. doi: 10.1111/j.1365-3083.1982.tb00648.x.
Monoclonal human B lymphoma cells were triggered in vitro to DNA synthesis with F(ab')2 fragments of rabbit IgG antibodies specific for different human immunoglobulin (Ig) light and heavy chains. The specificity of the responses corresponded to surface Ig (sIg) present on tumour cells. Anti-mu chain, anti-delta chain, and antibodies to light chains were found to mediate this effect. However, to induce DNA synthesis, the tumour promotor 12-O-tetradecanoyl-phorbol-13-acetate (TPA) was usually required. TPA alone induced cytoplasmic protrusions and increases in cellular volume. These observations should provide new opportunities to study the triggering of B cells with anti-Ig in well-defined cell populations.
用人源单克隆B淋巴瘤细胞在体外与兔IgG抗体的F(ab')2片段共同培养，这些抗体对不同的人免疫球蛋白（Ig）轻链和重链具有特异性，可引发细胞的DNA合成。反应的特异性与肿瘤细胞表面存在的表面Ig（sIg）相对应。发现抗μ链、抗δ链以及轻链抗体均可介导这种效应。然而，通常需要肿瘤促进剂12-O-十四烷酰佛波醇-13-乙酸酯（TPA）来诱导DNA合成。单独使用TPA可诱导细胞质突起并使细胞体积增大。这些观察结果将为研究在明确的细胞群体中用抗Ig触发B细胞提供新的机会。
