TPA（12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯）对人外周血B淋巴细胞的激活与分化作用
TPA (12-O-tetradecanoyl-phorbol-13-acetate) activation and differentiation of human peripheral B lymphocytes.
作者信息
Aman P, Gordon J, Klein G
出版信息
Immunology. 1984 Jan;51(1):27-34.
The effect of the tumour-promoting phorbol ester TPA (12-O-tetradecanoyl-phorbol-13-acetate) on normal human peripheral blood and tonsil B lymphocytes was investigated. A strong DNA-synthesis response with the maximum at day 4 was detected. This response was, however, inhibited by increasing concentrations of serum in the medium. The membrane Ig expression was changed with a rapid decrease in IgD expression and a slower decrease in IgM and IgG expression. TPA-induced Ig secretion was detected in 12 out of 22 tested donors and the response was found to be independent of T cells and macrophages. The expression of four monoclonal antibody-detected B cell activation and differentiation markers, B1, B2, LB1 and BB1, was followed. The results indicate activation and differentiation of the B cells.
研究了促肿瘤佛波酯TPA（12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯）对正常人外周血和扁桃体B淋巴细胞的作用。检测到强烈的DNA合成反应，在第4天达到最大值。然而，培养基中血清浓度的增加会抑制这种反应。膜免疫球蛋白（Ig）表达发生变化，IgD表达迅速下降，IgM和IgG表达下降较慢。在22名受试供体中的12名检测到TPA诱导的Ig分泌，并且发现该反应独立于T细胞和巨噬细胞。跟踪了四种单克隆抗体检测的B细胞活化和分化标志物B1、B2、LB1和BB1的表达。结果表明B细胞被激活并分化。
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