Triebel F, Gluckman J C, Debre P, Charron D J
Immunology. 1984 Dec;53(4):651-7.
PHA-stimulated lymphocyte supernatants (P-SUP) containing colony promoting activity (CPA) and interleukin-2 (IL-2) were found to drive T-lymphocyte precursor cells (OKT4-OKT8- blood cells) into colonies of mature T cells in agar. Ammonium sulphate precipitation followed by gel filtration on Sephadex G-75 showed that both IL-2 activity and CPA coeluted in the 21,000 MW fraction. Chromatofocusing on a PBE 94 gel equilibrated between pH 7.4 and 4.2 showed that CPA coeluted with the two main peaks of IL-2 (pI 7.2, pI 6.7). Adsorption of IL-2 activity by more than 97% on different T-cell lines did not completely abolish CPA. These results suggest that other(s) factor(s) with the same biochemical characteristics as IL-2 play a role in the induction of growth and differentiation of immature T-cell precursors.
含有集落促进活性(CPA)和白细胞介素-2(IL-2)的PHA刺激淋巴细胞上清液(P-SUP)被发现可促使T淋巴细胞前体细胞(OKT4-OKT8-血细胞)在琼脂中形成成熟T细胞集落。硫酸铵沉淀后在Sephadex G-75上进行凝胶过滤表明,IL-2活性和CPA在21,000分子量级分中共同洗脱。在pH 7.4和4.2之间平衡的PBE 94凝胶上进行色谱聚焦表明,CPA与IL-2的两个主峰(pI 7.2,pI 6.7)共同洗脱。不同T细胞系对IL-2活性的吸附超过97%并未完全消除CPA。这些结果表明,具有与IL-2相同生化特性的其他因子在未成熟T细胞前体的生长和分化诱导中起作用。