Lankas G R, Baxter C S, Christian R T
J Toxicol Environ Health. 1978 Jan;4(1):37-41. doi: 10.1080/15287397809529642.
Linear alkanes of specific chain length between 6 and 16 carbon atoms, an aryl derivative of dodecane, and a phorbol diester were tested in a cell culture system for relative ability to enhance mutagenesis induced by a chemical carcinogen, methylazoxymethanol acetate (MAM). Mutation frequencies at the ouabain-resistance locus were measured. Results indicated an excellent correlation between the relative activities of the above compounds in enhancing mutagenesis in the in vitro culture system and their tumor-promoting activities in mouse skin. None of the compounds tested showed mutagenic activity per se, further lending support to the theory that promoters act via derepression of latent carcinogen-induced damage to the genome.
在细胞培养系统中，对碳原子数在6至16之间的特定链长的直链烷烃、十二烷的芳基衍生物和佛波醇二酯进行了测试，以评估它们增强化学致癌物乙酸甲基偶氮甲醇（MAM）诱导的诱变作用的相对能力。测量了哇巴因抗性位点的突变频率。结果表明，上述化合物在体外培养系统中增强诱变作用的相对活性与其在小鼠皮肤中的促肿瘤活性之间存在良好的相关性。所测试的化合物本身均未显示出诱变活性，这进一步支持了启动子通过解除潜在致癌物诱导的基因组损伤的抑制作用来发挥作用的理论。
