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C4多态性:使用单克隆抗体区分C4A和C4B基因座产物。

C4 polymorphism: use of a monoclonal antibody to distinguish C4A and C4B locus products.

作者信息

O'Neill G J

出版信息

Vox Sang. 1984;47(5):362-5. doi: 10.1111/j.1423-0410.1984.tb04140.x.

DOI:10.1111/j.1423-0410.1984.tb04140.x
PMID:6334403
Abstract

A monoclonal antibody reactive against C4B locus products was used in a passive immunoblotting technique to distinguish C4B from C4A electrophoretic variants. The technique is simple and has the advantage of being able to distinguish clearly those C4B variants which may be either difficult to define by conventional hemolytic assay alone or which would normally be designated as C4B products on account of their lack of hemolytic function. Patterns detected by immunoblotting can be compared directly with patterns obtained by immunofixation with anti-C4 from the same gel.

摘要

一种针对C4B基因座产物的单克隆抗体被用于被动免疫印迹技术,以区分C4B和C4A电泳变体。该技术简单,其优点是能够清楚地区分那些仅通过传统溶血试验难以定义的C4B变体,或者那些由于缺乏溶血功能而通常被指定为C4B产物的变体。免疫印迹检测到的模式可以直接与同一凝胶上用抗C4进行免疫固定获得的模式进行比较。

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引用本文的文献

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