Alonso M C, Solana R, Torres A, de la Fuente M, Peña J
Allergol Immunopathol (Madr). 1984 Sep-Oct;12(5):363-7.
The study of patients with T cell lymphoproliferative disorders often demonstrates a reduced response to mitogens. The T cell lymphoma in AKR mice has been considered a classic experimental model of T cell leukaemias. In this paper ten spontaneous lymphomas from AKR mice were characterized individually in vitro for PHA reactivity. After 60 h. of culture, spontaneous 3H-Thymidine uptake by nylon wool purified spleen cells from individual lymphomas oscillated between 4.724 and 81.125 cpm. These nylon wool purified leukemic cells were not responsive to PHA (S.I. less than 2.5). When these cells were cocultured with an adequate macrophage concentration in the presence of PHA, all could be considered responsive to the mitogen (S.I. greater than 2.5). Results indicate that AKR leukemic cells are responsive to PHA in an absolutely macrophage dependent way.
对T细胞淋巴增殖性疾病患者的研究常常表明其对丝裂原的反应降低。AKR小鼠中的T细胞淋巴瘤被认为是T细胞白血病的经典实验模型。在本文中，对来自AKR小鼠的10个自发性淋巴瘤进行了体外PHA反应性的个体特征分析。培养60小时后，来自各个淋巴瘤的尼龙毛纯化脾细胞的自发性3H-胸腺嘧啶核苷摄取量在4.724至81.125 cpm之间波动。这些尼龙毛纯化的白血病细胞对PHA无反应（刺激指数小于2.5）。当这些细胞在PHA存在下与适当浓度的巨噬细胞共培养时，所有细胞都可被认为对丝裂原具有反应（刺激指数大于2.5）。结果表明，AKR白血病细胞对PHA的反应绝对依赖于巨噬细胞。
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