Eiklid K, Olsnes S
J Immunol. 1983 Jan;130(1):380-4.
The lethal effect to rabbits and mice of Shigella dysenteriae toxin and the ability of the toxin to induce fluid accumulation in rabbit ileal loops were studied in relation to the cytotoxic activity. The relative concentrations of the three activities were approximately the same in a crude toxin preparation and in purified, electrophoretically homogenous toxin. The cytotoxic and lethal activities eluted identically from a high pressure liquid chromatography column and migrated at the same rate in polyacrylamide gel electrophoresis under nondenaturing conditions. The cytotoxic, lethal, and enterotoxic activities were inactivated to essentially the same extent upon incubation for few minutes at 80 degrees C and upon treatment with urea. Graded precipitation of Shigella toxin with different amounts of an antiserum to Shigella toxin in each case removed essentially the same fraction of the cytotoxic, the lethal, and the enterotoxic activity. The data indicate that one molecular entity is responsible for the three biologic effects of Shigella toxin studied. After i.v. injection, the LD50 dose was estimated to be 2.2 ng/kg in rabbits and 450 ng/kg in mice.
研究了痢疾志贺氏菌毒素对家兔和小鼠的致死作用，以及该毒素在兔回肠袢中诱导液体蓄积的能力，并将其与细胞毒活性相关联。在粗毒素制剂以及纯化的、电泳均一的毒素中，这三种活性的相对浓度大致相同。细胞毒活性和致死活性从高压液相色谱柱上洗脱时完全相同，并且在非变性条件下于聚丙烯酰胺凝胶电泳中迁移速率相同。细胞毒、致死和肠毒素活性在80℃孵育几分钟以及用尿素处理后，失活程度基本相同。用不同量的痢疾志贺氏菌毒素抗血清对痢疾志贺氏菌毒素进行分级沉淀，每种情况下均去除了基本相同比例的细胞毒、致死和肠毒素活性。数据表明，一种分子实体负责所研究的痢疾志贺氏菌毒素的三种生物学效应。静脉注射后，家兔的半数致死剂量估计为2.2 ng/kg，小鼠为450 ng/kg。
