Tanaka M, Kato K
Cancer. 1983 Jan 1;51(1):61-4. doi: 10.1002/1097-0142(19830101)51:1<61::aid-cncr2820510115>3.0.co;2-8.
The concentration of ferritin in serum and leukemic blasts were measured with the improved method of enzyme immunoassay for ferritin which uses beta-D-galactosidase from Escherichia coli. Precise measurement of ferritin was possible for concentration as low as 0.2 ng/ml serum. A good correlation (correlation coefficient, 0.946) existed between the ferritin radioimmunoassay and enzyme immunoassay. The serum ferritin levels in healthy males were 97.2 +/- 46.7 ng/ml, whereas those in healthy females were 53.5 +/- 27.1 ng/ml. Serum ferritin level was markedly increased (698 +/- 447 ng/ml) in sera of 36 patients with various types of acute leukemia, of which acute monocytic leukemia showed the highest serum ferritin concentrations (1156 +/- 324 ng/ml). Leukemic blasts from 24 patients with acute leukemia contained markedly elevated concentrations of ferritin (64.3 +/- 38.7 ng/10(6) cells) as compared with the ferritin concentration in normal leukocytes (7.1 +/- 2.8 ng/10(6) cells). The blasts cells from the patients with acute monocytic leukemia showed the highest ferritin levels (105 +/- 26.6 ng/10(6) cells).
采用改进的铁蛋白酶免疫测定法，利用来自大肠杆菌的β-D-半乳糖苷酶，测定血清和白血病原始细胞中铁蛋白的浓度。对于低至0.2 ng/ml血清的铁蛋白浓度，均可进行精确测量。铁蛋白放射免疫测定法与酶免疫测定法之间存在良好的相关性（相关系数为0.946）。健康男性的血清铁蛋白水平为97.2±46.7 ng/ml，而健康女性为53.5±27.1 ng/ml。36例各类急性白血病患者血清中铁蛋白水平显著升高（698±447 ng/ml），其中急性单核细胞白血病患者的血清铁蛋白浓度最高（1156±324 ng/ml）。与正常白细胞中铁蛋白浓度（7.1±2.8 ng/10⁶细胞）相比，24例急性白血病患者的白血病原始细胞中铁蛋白浓度显著升高（64.3±38.7 ng/10⁶细胞）。急性单核细胞白血病患者的原始细胞中铁蛋白水平最高（105±26.6 ng/10⁶细胞）。
