用灵敏的酶免疫测定法测定正常成年人尿液和血清中的铁蛋白。

Determination of ferritin in urine and in serum of normal adults with a sensitive enzyme immunoassay.

作者信息

Ishikawa K, Narita O, Saito H, Kato K

出版信息

Clin Chim Acta. 1982 Aug 4;123(1-2):73-81. doi: 10.1016/0009-8981(82)90115-2.

Abstract

We have determined ferritin in both urine and serum of normal subjects by means of a sensitive sandwich-type enzyme immunoassay method. The assay system consisted of an antibody Fab'-beta-D-galactosidase conjugate and an antibody F(ab')2-immobilized solid phase. The lowest concentrations of ferritin detectable with the present method were 0.2 and 1 ng/ml in urine and in serum, respectively. There was a high correlation between the levels of ferritin in urine and those in serum (r = 0.79), and the geometric mean level in urine of normal males (4.1 ng/ml) was significantly higher than that of normal females (1.8 ng/ml) as was observed in their corresponding levels in serum. These results indicate that urine ferritin in normal subjects may also reflect body iron stores, and that the assay of urine ferritin may provide a useful means of evaluating iron stores.

摘要

我们采用一种灵敏的夹心型酶免疫测定法，测定了正常受试者尿液和血清中的铁蛋白。该测定系统由抗体Fab'-β-D-半乳糖苷酶结合物和抗体F(ab')2固定化固相组成。用本方法可检测到的尿液和血清中铁蛋白的最低浓度分别为0.2和1 ng/ml。尿液中铁蛋白水平与血清中铁蛋白水平之间存在高度相关性（r = 0.79），正常男性尿液中的几何平均水平（4.1 ng/ml）显著高于正常女性（1.8 ng/ml），血清中相应水平也有此现象。这些结果表明，正常受试者尿液中的铁蛋白也可能反映体内铁储存情况，且尿液铁蛋白测定可能为评估铁储存提供一种有用的方法。