Bogosian G, Haydock P V, Somerville R L
J Bacteriol. 1983 Feb;153(2):1120-3. doi: 10.1128/jb.153.2.1120-1123.1983.
Escherichia coli cells harboring a non-attenuated trp-lac operon fusion were used to evaluate the effects of indolmycin on the initiation of transcription at the trp promoter. Indolmycin caused repression in trpR+ strains and in trpR deletion mutants, although higher effector concentrations were required in the latter situation. Plasmid-mediated elevation in tryptophanyl-tRNA synthetase reversed the inhibitory effect of indolmycin. Indolmycin did not facilitate the binding of purified Trp repressor protein to trp operator DNA.
携带非弱化型色氨酸-乳糖操纵子融合基因的大肠杆菌细胞被用于评估吲哚霉素对色氨酸启动子转录起始的影响。吲哚霉素在色氨酸阻遏蛋白基因（trpR）阳性菌株和trpR缺失突变体中均能引起抑制作用，尽管在后一种情况下需要更高浓度的效应物。质粒介导的色氨酰-tRNA合成酶水平升高可逆转吲哚霉素的抑制作用。吲哚霉素不能促进纯化的色氨酸阻遏蛋白与色氨酸操纵基因DNA的结合。
