van der Valk P, van den Besselaar-Dingjan G, Daha M R, Meijer C J
J Clin Pathol. 1983 Jan;36(1):44-50. doi: 10.1136/jcp.36.1.44.
Fifteen cases of large-cell lymphoma, diagnosed as centroblastic (5), B-immunoblastic (5) or true histiocytic (5). lymphoma and one case of malignant histiocytosis were studied with monoclonal antibodies. Each diagnosis was based on morphological as well as marker studies. A panel of monoclonal and heterologous antibodies against T lymphocyte differentiation antigens (Leul, Leu2a, Leu3a, OKT4, OKT8, TA1), B lymphocyte subsets (BA1, BA2, HLA-DR, alpha C3b receptor antiserum, surface immunoglobulins), the common acute lymphoblastic leukaemia antigen (CALLA), monocytes/macrophages (OKM1, anti-human monocyte 1, TA1, Mac1, HLA-DR, anti-C3b receptor), myeloid cells (VIM-D5, elastase, OKM1) and the cells of the Langerhans cell/interdigitating reticulum cell series (OKT6, NA1/34). The results show a specific staining pattern for true histiocytic lymphoma (histiocytic sarcoma). Centroblastic and B-immunoblastic lymphomas showed gradual differences with mostly strong staining for HLA-DR and weak with anti C3b receptor for B-immunoblastic lymphomas in contrast to centroblastic lymphomas. Staining with BA1 and BA2 indicated immunological heterogeneity in these lymphomas. The number of admixed cells was usually low with few B cells and a shift in the ratio helper/inducer to suppressor/cytotoxic T cells in favour of the suppressor/cytotoxic subset.
对15例大细胞淋巴瘤（诊断为中心母细胞性5例、B免疫母细胞性5例或真性组织细胞性5例）及1例恶性组织细胞增多症患者进行了单克隆抗体研究。每项诊断均基于形态学及标志物研究。使用了一组针对T淋巴细胞分化抗原（Leul、Leu2a、Leu3a、OKT4、OKT8、TA1）、B淋巴细胞亚群（BA1、BA2、HLA - DR、αC3b受体抗血清、表面免疫球蛋白）、常见急性淋巴细胞白血病抗原（CALLA）、单核细胞/巨噬细胞（OKM1、抗人单核细胞1、TA1、Mac1、HLA - DR、抗C3b受体）、髓样细胞（VIM - D5、弹性蛋白酶、OKM1）以及朗格汉斯细胞/交错突网状细胞系列细胞（OKT6、NA1/34）的单克隆和异源抗体。结果显示真性组织细胞性淋巴瘤（组织细胞肉瘤）有特定的染色模式。中心母细胞性和B免疫母细胞性淋巴瘤表现出逐渐的差异，B免疫母细胞性淋巴瘤大多对HLA - DR呈强染色，而与中心母细胞性淋巴瘤相比，对抗C3b受体呈弱染色。用BA1和BA2染色表明这些淋巴瘤存在免疫异质性。混合细胞数量通常较少，B细胞很少，辅助/诱导性T细胞与抑制/细胞毒性T细胞的比例发生偏移，有利于抑制/细胞毒性亚群。
