Tsokos M, Howard R, Costa J
Lab Invest. 1983 Feb;48(2):148-55.
Paraffin-embedded sections of 11 alveolar and 12 embryonal rhabdomyosarcomas, 12 lymphomas, five neuroblastomas, five extraskeletal neoplasms resembling Ewing's sarcoma, and six epithelial tumors were tested for immunoreactivity against myosin, myoglobin, and isozymes BB and MM of creatine kinase with a peroxidase-antiperoxidase method. Of the 23 cases of rhabdomyosarcomas 17 were positive for at least three of the antigenic determinants. In contrast, the other investigated tumors were consistently negative for all markers, with the exception of breast and prostatic carcinomas. Our results establish that the presence of three or four of the above markers in a tumor is strongly suggestive of a rhabdomyosarcoma and helpful in the distinction of alveolar and embryonal rhabdomyosarcomas from lymphomas, neuroblastomas, and extraskeletal neoplasms resembling Ewing's sarcoma.
采用过氧化物酶-抗过氧化物酶法，对11例肺泡型横纹肌肉瘤、12例胚胎型横纹肌肉瘤、12例淋巴瘤、5例神经母细胞瘤、5例类似尤文肉瘤的骨外肿瘤以及6例上皮性肿瘤的石蜡包埋切片进行检测，以观察其对肌球蛋白、肌红蛋白以及肌酸激酶同工酶BB和MM的免疫反应性。在23例横纹肌肉瘤病例中，17例至少对三种抗原决定簇呈阳性反应。相比之下，除乳腺癌和前列腺癌外，其他所研究的肿瘤对所有标记物均持续呈阴性反应。我们的结果表明，肿瘤中存在上述三种或四种标记物强烈提示为横纹肌肉瘤，有助于将肺泡型和胚胎型横纹肌肉瘤与淋巴瘤、神经母细胞瘤以及类似尤文肉瘤的骨外肿瘤区分开来。
