Garvin A J, Surrette F, Hintz D S, Rudisill M T, Sens M A, Sens D A
Am J Pathol. 1985 Nov;121(2):298-310.
Skeletal muscle differentiation within a Wilms' tumor is a well-documented histopathologic entity thought to occur at a relatively low incidence and influence prognosis. A serum-free hormonally defined growth medium has been developed, allowing the long-term growth of the skeletal muscle component of Wilms' tumors. Eight Wilms' tumors have been grown under these conditions. Three cases grew a homogeneous population of cells which ultrastructurally displayed all stages of myogenesis through myotubule formation. They also possessed immunoreactivity for skeletal muscle myosin and myoglobin and synthesized the M and B subunits of creatine kinase. Of interest was the finding that the ability to yield skeletal muscle cultures was limited to those cases which exhibited skeletal muscle fibers in vivo. This technique is also a very sensitive marker for identifying Wilms' tumors possessing a myoid component. A second serum-free hormonally defined medium has also been developed that supports the long-term culture of a unique cell type from Wilms' tumors which contain a myoid component. These cells are spindle-shaped and exhibit all of the characteristics of early myoblasts.
肾母细胞瘤内的骨骼肌分化是一种有充分文献记载的组织病理学实体，据认为其发生率相对较低且会影响预后。现已开发出一种无血清的激素限定生长培养基，可使肾母细胞瘤的骨骼肌成分长期生长。在这些条件下培养了8例肾母细胞瘤。3例培养出了均一的细胞群体，这些细胞在超微结构上显示出从成肌细胞到肌管形成的所有肌生成阶段。它们还对骨骼肌肌球蛋白和肌红蛋白具有免疫反应性，并合成肌酸激酶的M和B亚基。有趣的是，发现能够培养出骨骼肌细胞的能力仅限于那些在体内表现出骨骼肌纤维的病例。该技术也是鉴定具有肌样成分的肾母细胞瘤的非常敏感的标志物。还开发了第二种无血清的激素限定培养基，该培养基支持来自含有肌样成分的肾母细胞瘤的一种独特细胞类型的长期培养。这些细胞呈纺锤形，具有早期成肌细胞的所有特征。
