Couch C B, Strittmatter W J
Cell. 1983 Jan;32(1):257-65. doi: 10.1016/0092-8674(83)90516-0.
The calcium-dependent fusion of cultured rat myoblasts to multinucleate myotubes appears to require the activity of a neutral metalloendoprotease at the time of fusion. Metalloendoprotease inhibitors and synthetic dipeptide substrates prevent myoblast fusion when added to fusion-competent myoblasts with the addition of calcium. Metalloendoprotease activity has been identified and partially characterized in myoblast membranes with a fluorogenic protease substrate, and is inhibited by the same compounds that prevent fusion.
培养的大鼠成肌细胞与多核肌管的钙依赖性融合似乎在融合时需要一种中性金属内蛋白酶的活性。当将金属内蛋白酶抑制剂和合成二肽底物添加到有融合能力的成肌细胞中并加入钙时，可阻止成肌细胞融合。已用一种荧光蛋白酶底物在成肌细胞膜中鉴定出金属内蛋白酶活性并对其进行了部分表征，且该活性受到与阻止融合相同的化合物的抑制。
