de Oliveira Alisson Rodrigo, de Miranda Soares Maria Albertina, Gomes Jose Rosa
Biomedical Science Post Graduate Program and Department of Structural, Genetic and Molecular Biology, Campus of Uvaranas, University of Ponta Grossa, Avenue Carlos Cavalcanti, 4748, Ponta Grossa, PR, 84030-900, Brazil.
Histochem Cell Biol. 2025 Aug 5;163(1):79. doi: 10.1007/s00418-025-02405-1.
There are pertinent studies on the relationship between matrix metalloproteinases (MMPs) and muscle cell differentiation in vitro, but currently, there are few in vivo studies exploring MMP expression in chicken muscle during different stages of differentiation. Therefore, we aimed to investigate in vivo whether MMP-2, MT1-MMP, and MMP-9 are expressed in the pectoral muscle during the fetal stages of Ross chicken development on days E11, E15, and E19. Our results demonstrated that, in contrast to earlier reports in vitro, myocyte fusion occurs on E11, while on E15 and E19, secondary muscle wave formation is occurring. MMP-2, on the other hand, appears to be expressed more than MT1-MMP throughout the evaluated days, whereas MMP-9 is not expressed at any point. Additionally, serine was discovered as an unexpected finding in the zymogram analysis. In conclusion, considering the experimental limitations of the present study, it was found that MMP-9 is not expressed during the evaluated ages of in vivo pectoral muscle development in Ross chickens, indicating a limited role for this enzyme in muscle differentiation during the fetal period. In contrast, the expression of MMP-2 and MT1-MMP is crucial, as these are detected from myocyte fusion at E11 to the development of myotubes observed at E19, reflecting their significant role in muscle differentiation within the Ross chicken lineage.
关于基质金属蛋白酶（MMPs）与体外肌肉细胞分化之间的关系已有相关研究，但目前，在体内研究鸡肌肉在不同分化阶段MMP表达的研究较少。因此，我们旨在研究在罗斯鸡胚胎发育的E11、E15和E19天的胎儿阶段，胸肌中是否表达MMP-2、MT1-MMP和MMP-9。我们的结果表明，与早期体外研究报告不同，肌细胞融合发生在E11天，而在E15和E19天，次级肌波形成正在发生。另一方面，在整个评估期间，MMP-2的表达似乎比MT1-MMP更多，而MMP-9在任何时间点均未表达。此外，在酶谱分析中意外发现了丝氨酸。总之，考虑到本研究的实验局限性，发现在罗斯鸡胸肌发育的评估年龄期间MMP-9未表达，表明该酶在胎儿期肌肉分化中的作用有限。相比之下，MMP-2和MT1-MMP的表达至关重要，因为从E11天的肌细胞融合到E19天观察到的肌管发育都能检测到它们，这反映了它们在罗斯鸡谱系肌肉分化中的重要作用。
