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酵母tRNAleu3基因a区域的突变，该突变允许转录，但消除剪接和5'端成熟。

Mutation in the a block of the yeast tRNAleu3 gene that allows transcription but abolishes splicing and 5'-end maturation.

作者信息

Mattoccia E, Baldi M I, Pande G, Ogden R, Tocchini-Valentini G P

出版信息

Cell. 1983 Jan;32(1):67-76. doi: 10.1016/0092-8674(83)90497-x.

DOI:10.1016/0092-8674(83)90497-x
PMID:6337723
Abstract

A three-base substitution mutant of the yeast tRNALeu3 has been constructed. The mutation, introduced through the use of a heptadecanucleotide as a site-specific mutagen, is localized in the anterior portion of the promoter and results in the inability to form a D stem. The mutant is active in transcription, but maturation of the 5' terminus and splicing are abolished. The results are discussed in the light of a recently proposed model for initiation of transcription of eucaryotic tRNA genes.

摘要

已构建出酵母tRNALeu3的一个三碱基替换突变体。该突变是通过使用十七聚核苷酸作为位点特异性诱变剂引入的，位于启动子的前部，导致无法形成D茎。该突变体在转录中具有活性，但5'末端的成熟和剪接被消除。根据最近提出的真核tRNA基因转录起始模型对结果进行了讨论。

相似文献

1
Mutation in the a block of the yeast tRNAleu3 gene that allows transcription but abolishes splicing and 5'-end maturation.酵母tRNAleu3基因a区域的突变，该突变允许转录，但消除剪接和5'端成熟。
Cell. 1983 Jan;32(1):67-76. doi: 10.1016/0092-8674(83)90497-x.
2
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Effect of intron mutations on processing and function of Saccharomyces cerevisiae SUP53 tRNA in vitro and in vivo.内含子突变对酿酒酵母SUP53 tRNA体外及体内加工与功能的影响。
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The yeast cloning vector YEp13 contains a tRNALeu3 gene that can mutate to an amber suppressor.酵母克隆载体YEp13含有一个可突变为琥珀抑制基因的tRNALeu3基因。
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引用本文的文献

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Mol Cell Biol. 1983 Nov;3(11):1996-2005. doi: 10.1128/mcb.3.11.1996-2005.1983.
2
Each element of the Drosophila tRNAArg gene split promoter directs transcription in Xenopus oocytes.果蝇tRNA精氨酸基因分裂启动子的每个元件都能在非洲爪蟾卵母细胞中指导转录。
Nucleic Acids Res. 1983 Dec 20;11(24):8677-90. doi: 10.1093/nar/11.24.8677.
3
Mutations affecting excision of the intron from a eukaryotic dimeric tRNA precursor.
影响真核生物二聚体tRNA前体中内含子切除的突变。
EMBO J. 1984 Jul;3(7):1573-80. doi: 10.1002/j.1460-2075.1984.tb02013.x.
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Transcription of tRNA gene fragments by HeLa cell extracts.HeLa细胞提取物对tRNA基因片段的转录
Mol Gen Genet. 1984;197(1):55-61. doi: 10.1007/BF00327922.
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The role of non-coding DNA sequences in transcription and processing of a yeast tRNA.非编码DNA序列在酵母tRNA转录和加工中的作用。
Nucleic Acids Res. 1983 Sep 10;11(17):5969-88. doi: 10.1093/nar/11.17.5969.
6
Generation of long read-through transcripts in vivo and in vitro by deletion of 3' termination and processing sequences in the human tRNAimet gene.通过缺失人tRNAimet基因中的3' 终止和加工序列在体内和体外生成长期通读转录本。
Nucleic Acids Res. 1984 Jan 25;12(2):1101-15. doi: 10.1093/nar/12.2.1101.
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Differential binding of a S. cerevisiae RNA polymerase III transcription factor to two promoter segments of a tRNA gene.酿酒酵母RNA聚合酶III转录因子与tRNA基因的两个启动子区段的差异结合。
EMBO J. 1984 Apr;3(4):847-53. doi: 10.1002/j.1460-2075.1984.tb01895.x.
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Structural alterations in mutant precursors of the yeast tRNALeu3 gene which behave as defective substrates for a highly purified splicing endoribonuclease.酵母tRNALeu3基因的突变前体中的结构改变，这些突变前体对于一种高度纯化的剪接内切核糖核酸酶而言表现为有缺陷的底物。
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