Setchell K D, Matsui A
Clin Chim Acta. 1983 Jan 7;127(1):1-17. doi: 10.1016/0009-8981(83)90070-0.
Routine and research techniques are outlined for the analysis of bile acids in serum. The basis of these techniques is the use of liquid-solid extraction and liquid-gel chromatography coupled with the measurement of bile acids by gas chromatography using high resolution glass capillary columns and mass spectrometry which provides increased sensitivity compared with conventional methods of measurement. Problems associated with the isolation and purification of bile acids from serum are discussed and rapid and flexible methods for their metabolic profiling are described. The advantages and applicability of these procedures are illustrated with examples of profiles of bile acids in the serum from normal subjects, patients with liver disease and a patient with an ileal resection.
概述了用于分析血清中胆汁酸的常规和研究技术。这些技术的基础是使用液固萃取和液-凝胶色谱法，并结合使用高分辨率玻璃毛细管柱的气相色谱法和质谱法来测量胆汁酸，与传统测量方法相比，质谱法具有更高的灵敏度。讨论了从血清中分离和纯化胆汁酸相关的问题，并描述了用于其代谢谱分析的快速灵活方法。通过正常受试者、肝病患者和回肠切除患者血清中胆汁酸谱的实例说明了这些程序的优点和适用性。
