Barnes S, Chitranukroh A
Ann Clin Biochem. 1977 Jul;14(4):235-9. doi: 10.1177/000456327701400163.
A simple and reproducible method using the non-ionic resin, Amberlite XAD-7, for the isolation of bile acids from serum by a batch procedure is described. Recoveries were greater than 95% for the non-sulphated bile acids and greater than 70% for the sulphate esters of bile acids. By using 1 g of resin, recoveries were independent of the mass (0.1-5 mumol) of the bile acid present. Up to 35 samples a day can be extracted without requiring dedication of the operator. When serum extracts were analysed by the 3alpha-hydroxysteroid dehydrogenase procedure for estimation of bile acids, virtually all the background fluorescence was eliminated. These extracts were also suitable for gas liquid chromatography, thin layer chromatography, and radioimmunoassay.
本文描述了一种使用非离子树脂Amberlite XAD - 7通过批量操作从血清中分离胆汁酸的简单且可重复的方法。非硫酸化胆汁酸的回收率大于95%，胆汁酸硫酸酯的回收率大于70%。使用1克树脂时，回收率与存在的胆汁酸质量（0.1 - 5微摩尔）无关。每天可提取多达35个样本，无需操作人员专门投入。当通过3α - 羟基类固醇脱氢酶法分析血清提取物以估算胆汁酸时，几乎消除了所有背景荧光。这些提取物也适用于气相色谱、薄层色谱和放射免疫分析。
