Hammond M M, Timoney P J
Cornell Vet. 1983 Jan;73(1):82-97.
An electron microscopic study was carried out on specimens of feces and intestinal contents from cases of canine gastroenteritis submitted to the Diagnostic Laboratory, New York State College of Veterinary Medicine, during 1979-1981. The majority of samples came from New York State and the Northeast with no marked shift in distribution over the three year period. Canine parvovirus was the major virus identified. In August and September 1980 there was an epidemic of canine gastroenteritis, with 247 samples received during this two month period alone, of which 48 percent were positive for canine parvovirus. Almost half of the total number of specimens examined were from dogs less than 6 months of age and well over 50 percent of these were parvovirus positive. In addition to canine parvovirus, three cases of coronavirus, two cases of rota-like virus and one case of astro-like virus were detected. Three dual infections with canine parvovirus and rota or astro-like virus were also confirmed. An unidentified virus-like particle with cubic symmetry was found in two specimens. The adoption of immunoelectron microscopy for the detection of canine parvovirus in March 1980 facilitated identification of this virus and greatly increased the sensitivity of the technique.
1979年至1981年期间，对提交至纽约州兽医学院诊断实验室的犬肠胃炎病例的粪便和肠道内容物标本进行了电子显微镜研究。大多数样本来自纽约州和东北部地区，三年间分布没有明显变化。犬细小病毒是鉴定出的主要病毒。1980年8月和9月发生了犬肠胃炎疫情，仅这两个月就收到247份样本，其中48%的样本犬细小病毒呈阳性。几乎一半的送检标本来自6个月龄以下的犬，其中超过50%的样本细小病毒呈阳性。除犬细小病毒外，还检测到3例冠状病毒感染、2例轮状样病毒感染和1例星状样病毒感染。还证实了3例犬细小病毒与轮状或星状样病毒的双重感染。在两份标本中发现了一种具有立方对称结构的未鉴定病毒样颗粒。1980年3月采用免疫电子显微镜检测犬细小病毒，有助于该病毒的鉴定，并大大提高了该技术的灵敏度。
