Kölbl S, Vogel I, Modli M, Gerstl F
Bundesanstalt für Virusseuchenbekämpfung bei Haustieren.
Berl Munch Tierarztl Wochenschr. 1990 Jul 1;103(7):232-6.
79 feces samples of dogs between 7 weeks till 13.5 years of age, showing clinical signs of a hemorrhagic gastroenteritis, were tested by a commercial ELISA (DiaSystems Canine Parvo, Tech-America) for Canine Parvovirus (CPV) and by two Latex-Agglutination tests for Rotavirus (30 probes were tested by Rota Screen, 49 samples by Slidex Rota-Kit 2, both tests from BioMerieux). All samples were also examined by electron microscopy. The results of the simultaneous investigations showed 28 times positive and 28 times negative for CPV (70.9%). In 93.7% the investigations for Rotavirus-infection showed identical results by the Latex-Agglutination and electron microscopy: 73 samples were negative, one case showed a positive reaction. In 4 feces samples Rotavirus could only be detected by the Latex-test. In one sample a double-infection (CPV/Rotavirus) could be observed by all methods, in two cases the double-infection was only found by using the Latex-Agglutination. No other viruses could be found by the electron microscope than those described above. The results and the performance of the methods are discussed and compared with the data of other authors.
对79份7周龄至13.5岁出现出血性肠胃炎临床症状的犬粪便样本，采用商业酶联免疫吸附测定法（DiaSystems犬细小病毒检测试剂盒，Tech - America公司）检测犬细小病毒（CPV），并采用两种乳胶凝集试验检测轮状病毒（30份样本用Rota Screen检测，49份样本用Slidex Rota - Kit 2检测，两种检测均来自生物梅里埃公司）。所有样本还通过电子显微镜进行检查。同步检测结果显示，CPV检测呈阳性28次，阴性28次（70.9%）。轮状病毒感染检测中，93.7%的乳胶凝集试验和电子显微镜检查结果一致：73份样本为阴性，1例呈阳性反应。在4份粪便样本中，轮状病毒仅通过乳胶试验检测到。在1份样本中，所有方法均检测到双重感染（CPV/轮状病毒），在2例中，仅通过乳胶凝集试验发现双重感染。电子显微镜未发现除上述病毒外的其他病毒。对结果及方法的性能进行了讨论，并与其他作者的数据进行了比较。
